Biomolecular NMR Assignments

, Volume 7, Issue 2, pp 175–178 | Cite as

Sequence-specific 1H, 13C and 15N NMR assignments of Cyclophilin A like protein from Piriformospora indica involved in salt stress tolerance

  • Dipesh Kumar Trivedi
  • Harshesh Bhatt
  • Atul Johri
  • Narendra Tuteja
  • Neel Sarovar BhaveshEmail author


Cyclophilins are omnipresent proteins found in eukaryotes and prokaryotes, with presence in cytoplasm as well as in nucleus. Primary role of Cyclophilins is of peptidyl-prolyl cis–trans isomerase, a molecular chaperon action. Here, we report sequence-specific 1H, 13C and 15N resonance assignments for a Cyclophilin A like protein from Piriformospora indica. This protein is up-regulated during salt stress conditions.


Piriformospora indica Salt stress Cyclophilin A 



This study is supported by grants to NT and NSB from Department of Science and Technology (DST), Government of India and ICGEB core funds. We thank Department of Biotechnology (DBT), Government of India for providing financial support for the 500 MHz and 700 MHz NMR spectrometers at the ICGEB, New Delhi and NII, New Delhi. We thank Prof. Virander S. Chauhan for his interest and support in establishing the NMR facility. DKT is a recipient of Department of Biotechnology (DBT) junior research fellowship. HB is a recipient of Council for Scientific and Industrial Research (CSIR) senior research fellowship.


  1. Bhavesh NS, Panchal SC, Hosur RV (2001) An efficient high-throughput resonance assignment procedure for structural genomics and protein folding research by NMR. Biochemistry 40:14727–14735CrossRefGoogle Scholar
  2. Das A, Kamal S, Shakil NA, Sherameti I, Oelmüller R, Dua M, Tuteja N, Johri AK, Varma A (2012) The root endophyte fungus Piriformospora indica leads to early flowering, higher biomass and altered secondary metabolites of the medicinal plant, Coleus forskohlii. Plant Signal Behav 7:103–112Google Scholar
  3. Davis TL, Walker JR, Campagna-Slater V, Finerty PJ Jr, Paramanathan R, Bernstein G, MacKenzie F, Tempel W, Ouyang H, Lee WH, Eisenmesser EZ, Dhe-Paganon S (2010) Structural and biochemical characterization of the human cyclophilin family of peptidyl-prolyl isomerases. PLoS Biol 8:e1000439CrossRefGoogle Scholar
  4. Keller R (2004) The computer aided resonance assignment tutorial, 1st edn. CANTINA Verlag. ISBN 3-85600-112-3Google Scholar
  5. Tuteja N (2007) Mechanisms of high salinity tolerance in plants. Meth Enzymol 428:419–438Google Scholar
  6. Verma S, Varma A, Rexer KH, Hassel A, Kost G, Sarbhoy A, Bisen P, Bütehorn B, Franken P (1998) Piriformospora indica, gen. et sp. nov., a new root-colonizing fungus. Mycologia 90:896–903CrossRefGoogle Scholar
  7. Waller F, Achatz B, Baltruschat H, Fodor J, Becker K, Fischer M, Heier T, Hückelhoven R, Neumann C, Wettstein DV, Franken P, Kogel K (2005) The endophytic fungus Piriformospora indica reprograms barley to salt-stress tolerance, disease resistance, and higher yield. PNAS 102:13386–13391ADSCrossRefGoogle Scholar
  8. Wang P, Heitman J (2005) The cyclophilins. Genome Biol 6:226CrossRefGoogle Scholar
  9. Wishart DS, Sykes BD (1994) The 13C chemical-shift index: a simple method for the identification of protein secondary structure using 13C chemical-shift data. J Biomol NMR 4:171–180CrossRefGoogle Scholar
  10. Xiong L, Schumaker KS, Zhu J (2002) Cell signaling during cold, drought, and salt stress. Plant Cell 14:165–183CrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media B.V. 2012

Authors and Affiliations

  • Dipesh Kumar Trivedi
    • 2
  • Harshesh Bhatt
    • 1
  • Atul Johri
    • 3
  • Narendra Tuteja
    • 2
  • Neel Sarovar Bhavesh
    • 1
    Email author
  1. 1.Structural and Computational Biology GroupInternational Centre for Genetic Engineering and Biotechnology (ICGEB)New DelhiIndia
  2. 2.Plant Molecular Biology GroupInternational Centre for Genetic Engineering and Biotechnology (ICGEB)New DelhiIndia
  3. 3.School of Life SciencesJawaharlal Nehru UniversityNew DelhiIndia

Personalised recommendations