Biomolecular NMR Assignments

, Volume 7, Issue 2, pp 149–153

1H, 15N and 13C backbone resonance assignments of the Kelch domain of mouse Keap1

  • Elio Cino
  • Jingsong Fan
  • Daiwen Yang
  • Wing-Yiu Choy
Article

DOI: 10.1007/s12104-012-9398-6

Cite this article as:
Cino, E., Fan, J., Yang, D. et al. Biomol NMR Assign (2013) 7: 149. doi:10.1007/s12104-012-9398-6

Abstract

Kelch-like ECH-associated Protein 1 (Keap1) is a multi-domain protein that functions as an inhibitor of the transcription factor nuclear factor E2-related factor 2 (Nrf2) in the cellular response to oxidative stress. Under normal conditions, Keap1 binds to Nrf2 via its C-terminal Kelch domain and the interaction ultimately leads to the ubiquitin-dependent degradation of Nrf2. It has been proposed that designing molecules to selectively disrupt the Keap1–Nrf2 interaction can be a potential therapeutic approach for enhancing the expression of cytoprotective genes. Here, we reported the 1H, 13C, and 15N backbone chemical shift assignments of the Kelch domain of mouse Keap1. Further, unlabeled Nrf2 peptide containing the Kelch-binding motif was added to the 15N-labeled Kelch sample. 1H–15N HSQC spectra of the protein in the absence and presence of an equimolar concentration of the Nrf2 peptide were presented. A significant number of resonance signals were shifted upon addition of the peptide, confirming the protein–peptide interaction. The results here will not just facilitate the further studies of the binding between Keap1 and Nrf2, it will also be valuable for probing interactions between the Kelch domain and small molecules, as well as a growing list of protein targets that have been identified recently.

Keywords

Oxidative stress response Keap1 Kelch domain Nrf2 Protein–protein interaction 

Copyright information

© Springer Science+Business Media B.V. 2012

Authors and Affiliations

  • Elio Cino
    • 1
  • Jingsong Fan
    • 2
  • Daiwen Yang
    • 2
  • Wing-Yiu Choy
    • 1
  1. 1.Department of BiochemistryThe University of Western OntarioLondonCanada
  2. 2.Department of Biological SciencesNational University of SingaporeSingaporeSingapore

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