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1H, 13C and 15N resonance assignments and second structure information of Gad m 1: a β-parvalbumin allergen from Atlantic cod (Gadus morhua)

Biomolecular NMR Assignments volume 7, pages 133–136 (2013)Cite this article

Abstract

Gad m 1 is the major allergen from Atlantic cod. It belongs to β-parvalbumin protein family and is characterized by the presence of two calcium-binding sites so called EF-hand motifs. β-Parvalbumins such as Gad m 1 are the most important fish allergens and their high cross-reactivity is the cause of the observed polysensitization to various fish species in allergic patients. Despite extensive efforts, the complete elucidation of β-parvalbumin-IgE complexes has not been achieved yet. Allergen structural studies are essential for the development of novel immunotherapy strategies, including vaccination with hypoallergenic derivatives and chimeric molecules. Here, we report for the first time the NMR study of a β-parvalbumin: Gad m 1. This report includes: 1H, 13C and 15N resonance assignments of Gad m 1 as well as the second structure information based on the 13C chemical shifts.

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Affiliations

  1. Centro Nacional de Ressonância Magnética, Instituto de Bioquímica Médica, Universidade Federal do Rio de Janeiro, Rio de Janeiro, Brazil

    A. H. Moraes, F. C. L. Almeida & A. P. Valente

  2. Department of Pathophysiology and Allergy Research, Medical University of Vienna, Vienna, Austria

    D. Ackerbauer, M. Kostadinova, M. Bublin & H. Breiteneder

  3. Christian Doppler Laboratory for Allergy Diagnosis and Therapy, University of Salzburg, Salzburg, Austria

    F. Ferreira

Authors
  1. A. H. Moraes
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  2. D. Ackerbauer
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  3. M. Kostadinova
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  4. M. Bublin
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  5. F. Ferreira
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  6. F. C. L. Almeida
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  7. H. Breiteneder
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  8. A. P. Valente
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Corresponding author

Correspondence to A. P. Valente.

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Moraes, A.H., Ackerbauer, D., Kostadinova, M. et al. 1H, 13C and 15N resonance assignments and second structure information of Gad m 1: a β-parvalbumin allergen from Atlantic cod (Gadus morhua). Biomol NMR Assign 7, 133–136 (2013). https://doi.org/10.1007/s12104-012-9393-y

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  • DOI: https://doi.org/10.1007/s12104-012-9393-y

Keywords

  • Gad m 1
  • β-parvalbumin
  • Allergen
  • NMR assignments
  • Secondary structure