1H, 13C and 15N NMR assignments of inactive form of P1 endolysin Lyz
- 120 Downloads
Lysozyme (Lyz) encoded by phage P1 is required for host cell lysis upon infection. Lyz has a N-terminal Signal Anchor Release (SAR) domain, responsible for its secretion into the periplasm and for its accumulation in a membrane tethered inactive form. Here, we report sequence-specific 1H, 13C and 15N resonance assignments for secreted inactive form of Lyz at pH 4.5.
KeywordsPhage P1 Endolysin Cell lysis
We thank Department of Biotechnology, Government of India for providing financial support for the 500 and 700 MHz NMR spectrometers at the ICGEB, New Delhi and NII, New Delhi. MK is a recipient of Indian Council for Medical Research (ICMR) junior research fellowship. ZJ is recipient of University Grants Commission (UGC) junior research fellowship. BKD is a recipient of Council for Scientific and Industrial Research (CSIR) junior research fellowship. This study is supported by grants to AA and NSB from DBT and ICGEB core funds. Prof. Ry Young kindly provided the overexpression constructs of Lyz and Lyzi. We thank Harshesh Bhatt for the software support. Initial work on the project was done by Arun K. Tharkeshwar.
- Keller R (2004) The computer aided resonance assignment tutorial, 1st edn, ISBN 3-85600-112-3, CANTINA VerlagGoogle Scholar
- Schmidt C, Velleman M, Arber W (1996) Three functions of bacteriophage P1 involved in cell lysis. J Bacteriol 178(4):1099–1104Google Scholar
- Young R (1992) Bacteriophage lysis: mechanism and regulation. Microbiol Rev 56(3):430–481Google Scholar