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1H, 13C and 15N assignments of a camelid nanobody directed against human α-synuclein

  • Anneleen Vuchelen
  • Elizabeth O’Day
  • Erwin De Genst
  • Els Pardon
  • Lode Wyns
  • Mireille Dumoulin
  • Christopher M. Dobson
  • John Christodoulou
  • Shang-Te Danny Hsu
Article

Abstract

Nanobodies are single chain antibodies that are uniquely produced in Camelidae, e.g. camels and llamas. They have the desirable features of small sizes (Mw < 14 kDa) and high affinities against antigens (Kd ~ nM), making them ideal as structural probes for biomedically relevant motifs both in vitro and in vivo. We have previously shown that nanobody binding to amyloidogenic human lysozyme variants can effectively inhibit their aggregation, the process that is at the origin of systemic amyloid disease. Here we report the NMR assignments of a new nanobody, termed NbSyn2, which recognises the C-terminus of the intrinsically disordered protein, human α-synuclein (aS), whose aberrant self-association is implicated in Parkinson’s disease.

Keywords

Camelid antibody Nanobody Alpha-synuclein Intrinsically disordered protein Parkinson’s disease 

Notes

Acknowledgments

AV is a student at the Free University of Brussels and receives funding from the Lifelong Learning Programme/Erasmus. EDG acknowledges receipt of a long-term EMBO Fellowship and a Marie Curie Intra European Fellowship. CMD and JC acknowledge funding from the Wellcome and Leverhulme Trusts. STDH is a recipient of a Human Frontier Science Program Long-term Fellowship (LT0798/2005) and is supported in part by the National Science Council of the Republic of China, Taiwan (NSC97-2917-1-564-102). We also thank the staff of the Biomolecular NMR Facility, Department of Chemistry, University of Cambridge, for their valuable assistance and for the use of the Facility.

Supplementary material

12104_2009_9182_MOESM1_ESM.doc (149 kb)
DOC 149 kb

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Copyright information

© Springer Science+Business Media B.V. 2009

Authors and Affiliations

  • Anneleen Vuchelen
    • 1
  • Elizabeth O’Day
    • 1
    • 4
  • Erwin De Genst
    • 1
  • Els Pardon
    • 2
  • Lode Wyns
    • 2
  • Mireille Dumoulin
    • 1
  • Christopher M. Dobson
    • 1
  • John Christodoulou
    • 1
    • 3
  • Shang-Te Danny Hsu
    • 1
  1. 1.Department of ChemistryUniversity of CambridgeCambridgeUK
  2. 2.Structural Biology BrusselsFree University of BrusselsBrusselsBelgium
  3. 3.Institute of Structural and Molecular Biology, Research Department of Structural and Molecular BiologyUCLLondonUK
  4. 4.Chemical Biology DepartmentHarvard UniversityCambridgeUSA

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