Abstract
We report the assignment of the 110 amino acid second calponin homology domain of human α-actinin-4. The two calponin homology domains of α-actinin combine to regulate F-actin binding.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Brakebusch C, Fassler R (2003) The integrin-actin connection, an eternal love affair. EMBO J 22:2324–2333. doi:10.1093/emboj/cdg245
Bramham J, Hidgkinson JL, Smith BO, Uhrin D, Barlow PN, Winder SJ (2002) Solution structure of the calponin CH domain and fitting to the 3D-helical reconstruction of F-actin:calponin. Structure 10:249–258. doi:10.1016/S0969-2126(02)00703-7
Burridge K, Chrzanowska-Wodnicka M (1996) Focal adhesions, contractility, and signaling. Annu Rev Cell Dev Biol 12:463–519. doi:10.1146/annurev.cellbio.12.1.463
Delaglio F, Grzesiek S, Vuister GW, Zhu G, Pfeifer J, Bax A (1995) NMRPipe: a multidimensional spectral processing system based on UNIX pipes. J Biomol NMR 6:277–293. doi:10.1007/BF00197809
Fraley TS, Tran TC, Corgan AM, Nash CA, Hao J, Critchley DR et al (2003) Phosphoinositide binding inhibits alpha-actinin bundling activity. J Biol Chem 278:24039–24045. doi:10.1074/jbc.M213288200
Fukami K, Sawada N, Endo T, Takenawa T (1996) Identification of a phosphatidylinositol 4,5-bisphosphate-binding site in chicken muscle alpha-actinin. J Biol Chem 271:2646–2650. doi:10.1074/jbc.271.1.355
Honda K, Yamada T, Endo R, Ino Y, Gotoh M, Tsuda H et al (1998) Actinin-4, a novel actin-bunding protein associated with cell motility and cancer invasion. J Cell Biol 140:1383–1393. doi:10.1083/jcb.140.6.1383
Ishida H, Borman MA, Ostrander J, Vogel HJ, McDonald JA (2008) Solution structure of the calponin homology (CH) domain from the smoothelin-like 1 protein: a unique apocalmodulin-binding mode and the possible role of the C-terminal type-2 CH-domain in smooth muscle relaxation. J Biol Chem 283:20569–20578. doi:10.1074/jbc.M800627200
Liu J, Taylor DW, Taylor KA (2004) A 3-D reconstruction of smooth muscle alpha-actinin by cryoEm reveals two different conformations at the actin-binding region. J Mol Biol 338:115–125. doi:10.1016/j.jmb.2004.02.034
Nikolopoulos SN, Spengler BA, Kisselbach K, Evans AE, Biedler JL, Ross RA (2000) The human non-muscle alpha-actinin protein encoded by the ACTN4 gene suppresses tumorigenicity of human neuroblastoma cells. Oncogene 19:380–386. doi:10.1038/sj.onc.1203310
Sun H, Dai H, Zhang J, Xianju J, Xiong S, Xu J et al (2006) Solution structure of calponin homology domain of Human MICAL-1. J Biomol NMR 36:295–300. doi:10.1007/s10858-006-9062-5
Vranken WF, Boucher W, Stevens TJ, Fogh RH, Pajon A, Llinas M et al (2005) The CCPN data model for NMR spectroscopy: development of a software pipeline. Proteins 59:687–696. doi:10.1002/prot.20449
Winder SJ (2003) Structural insights into actin-binding, branching and bundling proteins. Curr Opin Cell Biol 15:14–22. doi:10.1016/S0955-0674(02)00002-9
Wishart DS, Bigam CG, Holm A, Hodges RS, Sykes BD (1995) 1H, 13C and 15N random coil NMR chemical shifts of the common amino acids I. Investigations of nearest-neighbor effects. J Biomol NMR 5:67–81. doi:10.1007/BF00227471
Acknowledgment
We thank Mr. Philip Clements for maintenance of the NMR spectrometer.
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Chen, HH., Murchland, I. & Booker, G.W. Assignment of the 1H, 13C and 15N resonances of the calponin homology-2 domain of α-actinin-4. Biomol NMR Assign 2, 195–197 (2008). https://doi.org/10.1007/s12104-008-9119-3
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s12104-008-9119-3