Skip to main content
Log in

1H, 15N, 13C resonance assignment of the AlgE6R1 subunit from the Azotobacter vinelandii mannuronan C5-epimerase

  • Article
  • Published:
Biomolecular NMR Assignments Aims and scope Submit manuscript

Abstract

Isotopically labelled, 13C/15N from of recombinant subunit of the first R-module from alginate C5-epimerase 6 (AlgE6R1) from Azotobacter vinelandii mannuronan C5-epimerase was produced. We report here the 1H, 15N, 13C resonance assignment of this subunit from AlgE6 epimerase.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Fig. 1

Similar content being viewed by others

References

  • Aachmann FL, Svanem BIG, Valla S et al (2005) NMR assignment of the R-module from the Azotobacter vinelandii Mannuronan C5-epimerase AlgE4. J Biomol NMR 31:259

    Article  Google Scholar 

  • Aachmann FL, Svanem BIG, Guntert P et al (2006) NMR structure of the R-module—a parallel beta-roll subunit from an Azotobacter vinelandii mannuronan C-5 epimerase. J Biol Chem 281:7350–7356

    Article  Google Scholar 

  • Campa C, Holtan S, Nilsen N et al (2004) Biochemical analysis of the processive mechanism for epimerization of alginate by mannuronan C-5 epimerase AlgE4. Biochem J 381:155–164

    Article  Google Scholar 

  • Ertesvåg H, Valla S (1999) The A modules of the Azotobacter vinelandii mannuronan-C-5-epimerase AlgE1 are sufficient for both epimerization and binding of Ca2+. J Bacteriol 181:3033–3038

    Google Scholar 

  • Ertesvåg H, Doseth B, Larsen B et al (1994) Cloning and expression of an Azotobacter vinelandii mannuronan C-5-epimerase gene. J Bacteriol 176:2846–2853

    Google Scholar 

  • Ertesvåg H, Høidal HK, Hals IK et al (1995) A family of modular type mannuronan C-5-epimerase genes controls alginate structure in Azotobacter vinelandii. Mol Microbiol 16:719–731

    Article  Google Scholar 

  • Ertesvåg H, Høidal HK, Schjerven H et al (1999) Mannuronan C-5-epimerases and their application for in vitro and in vivo design of new alginates useful in biotechnology. Metab Eng 1:262–269

    Article  Google Scholar 

  • Hartmann M, Holm OB, Johansen GA et al (2002) Mode of action of recombinant Azotobacter vinelandii mannuronan C-5 epimerases AlgE2 and AlgE4. Biopolymers 63:77–88

    Article  Google Scholar 

  • Keller RLJ (2004) Optimizing the process of nuclear magnetic resonance spectrum analysis and computer aided resonance assignment. Cantina Verlag, Goldau

  • Zhang HY, Neal S,Wishart DS (2003) RefDB: a database of uniformly referenced protein chemical shifts. J Biomol NMR 25:173–195

    Article  Google Scholar 

Download references

Acknowledgements

This work was financed by the Norwegian Research Council (NFR) via KMB project. We wish to thank Reinhard Wimmer for technical assistance.

Author information

Authors and Affiliations

Authors

Corresponding author

Correspondence to Finn L. Aachmann.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Aachmann, F.L., Skjåk-Bræk, G. 1H, 15N, 13C resonance assignment of the AlgE6R1 subunit from the Azotobacter vinelandii mannuronan C5-epimerase. Biomol NMR Assign 2, 123–125 (2008). https://doi.org/10.1007/s12104-008-9101-0

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s12104-008-9101-0

Keywords

Navigation