Abstract
Ras homologue enriched in brain (Rheb) is a small GTPase that plays an important role in tuberous sclerosis. Here we present the backbone assignments of activated rRheb in complex with the non-hydrolisable GTP analogue GppNHp. These assignments now provide a basis for the analysis of rRheb’s interaction with putative effectors in order to further elucidate the physiological function of this GTPase and its role in the regulation of neuronal cell volume as well as in tuberous sclerosis.
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Acknowledgements
We are extremely grateful to Gregor Barchan, Martin Gartmann, and Hans-Jochen Hauswald for expert technical help. This work was supported by a DFG grant (SFB 642, A6). The 750 MHz spectra were recorded at the SON NMR Large Scale Facility in Utrecht, which is funded by the European Union project “EU-NMR-European Network of Research Infrastructures for Providing Access & Technological Advancement in Bio-NMR” (FP-2005-RII3-Contract-no. 026145). Furthermore, R. S. gratefully recognizes generous support from the BMBF, FCI, Proteincenter (NRW center of excellence), and RUBiospek. We are grateful to Prof. Dr. von Kiedrowski for providing generous access to the DRX600.
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Schwarten, M., Berghaus, C., Heumann, R. et al. Sequence-specific 1H, 13C, and 15N backbone assignment of the activated 21 kDa GTPase rRheb. Biomol NMR Assign 1, 105–108 (2007). https://doi.org/10.1007/s12104-007-9030-3
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DOI: https://doi.org/10.1007/s12104-007-9030-3