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NMR assignment of the apo-form of a Desulfovibrio gigas protein containing a novel Mo–Cu cluster

Biomolecular NMR Assignments volume 1, pages 81–83 (2007)Cite this article

Abstract

We report the 98% assignment of the apo-form of an orange protein, containing a novel Mo–Cu cluster isolated from Desulfovibrio gigas. This protein presents a region where backbone amide protons exchange fast with bulk solvent becoming undetectable. These residues were assigned using 13C-detection experiments.

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References

  • Bermel W, Bertini I, Felli IC, Piccioli M, Pierattelli R (2006) 13C-detected protonless NMR spectroscopy of proteins in solution. Prog NMR Spectrosc 48:25–45

    Article  Google Scholar 

  • Bursakov SA, Gavel OY, Di Rocco G, Lampreia J, Calvete J, Pereira AS, Moura JJG, Moura I (2004) Antagonists Mo and Cu in a heterometallic cluster present on a novel protein (orange protein) isolated from Desulfovibrio gigas. J Inorg Biochem 98:833–884

    Article  Google Scholar 

  • Cort JR, Yee A, Edwards AM, Arrowsmith CH, Kennedy MA (2000) NMR structure determination and structure-based functional characterization of conserved hypothetical protein MTH1175 from Methanobacterium thermoautotrophicum. J Struct Funct Genomics 1:15–25

    Article  Google Scholar 

  • Etezady-Esfarjani T, Herrmann T, Peti W, Klock HE, Lesley SA, Wüthrich K (2004) NMR structure determination of the hypothetical protein TM1290 from Thermotoga maritima using automated NOESY analysis. J Biomol NMR 29:403–406

    Article  Google Scholar 

  • Ferentz AE, Wagner G (2000) NMR spectroscopy: a multifaceted approach to macromolecular structure. Q Rev Biophys 33:29–65

    Article  Google Scholar 

  • George GN, Pickering IJ, Yu EY, Prince RC, Bursakov SA, Gavel OY, Moura I, Moura JJG (2000) A novel protein-bound copper-molybdenum cluster. JACS 122:8321–8322

    Article  Google Scholar 

  • Scholten JC, Culley DE, Brockman FJ, Wu G, Zhang W (2007) Evolution of the syntrophic interaction between Desulfovibrio vulgaris and Methanosarcina barkeri: Involvement of an ancient horizontal gene transfer. Biochem Biophys Res Commun 352:48–54

    Article  Google Scholar 

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Acknowledgements

This work was supported by a research project from the Fundação para a Ciência e Tecnologia (POCI/QUI/55350/2004). SRP, AGD and MSC would like to thank the Fundação para a Ciência e Tecnologia for financial support (POCI/SFRH/BPD/14067/2003, POCI/QUI/55350/2004, POCI/SFRH/BPD/24150/2005, respectively). NMR experiments were run at the CERM EU-NMR Research Infrastructure (Contract n° 026145), and SRP would like to thank the EU-NMR for the financial support during her visits to CERM.

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  1. REQUIMTE/CQFB, Departamento de Química, Faculdade de Ciências e Tecnologia, Universidade Nova de Lisboa, Caparica, 2829-516, Portugal

    Sofia R. Pauleta, Américo G. Duarte, Marta S. Carepo, Alice S. Pereira, Pedro Tavares, Isabel Moura & José J. G. Moura

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  1. Sofia R. Pauleta
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  2. Américo G. Duarte
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  3. Marta S. Carepo
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  4. Alice S. Pereira
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  5. Pedro Tavares
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  6. Isabel Moura
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  7. José J. G. Moura
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Correspondence to Sofia R. Pauleta.

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Pauleta, S.R., Duarte, A.G., Carepo, M.S. et al. NMR assignment of the apo-form of a Desulfovibrio gigas protein containing a novel Mo–Cu cluster. Biomol NMR Assign 1, 81–83 (2007). https://doi.org/10.1007/s12104-007-9022-3

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  • DOI: https://doi.org/10.1007/s12104-007-9022-3

Keywords

  • NMR assignment
  • Carbon detection
  • Novel Mo–Cu cluster
  • Desulfovibrio