Urine Flow Cytometry in the Diagnosis of Urinary Tract Infection
- 125 Downloads
To determine the cut-off value of bacteria with urine flow cytometer in diagnosing urinary tract infection.
From a total of 546 patients admitted to the hospital with urinary tract infection (UTI), two urine samples were obtained for each patient. Urine samples in sterile containers were divided into two; first for urine culture and second for simultaneous measurement with UF-1000i flow cytometry device. The presence of a single type of bacteria over 103 CFU/mL in urine culture was accepted as UTI.
Of 546 patients, 210 (38.5%) were boys and 336 (61.5%) were girls. There was no growth in 489 (89.5%) of the urine specimens and 57 (10.4%) samples showed growth (>103 CFU/ml). A threshold of 10 bacteria/μL in flow cytometry (sensitivity = 100%) showed the best compatibility with culture. Diagnostic values in terms of sensitivity (100%), specificity (43.5%), negative predictive value (100%) and positive predictive value (17.7%) were satisfactory.
The UF-1000i flow cytometer can give results quickly and exclude UTI using the determined number of cut-off bacteria at low cost. Thus, if Sysmex UF-1000i is used, quick and accurate results can be obtained and unnecessary laboratory tests can be prevented. Also, patient convenience can be increased.
KeywordsChild Flow cytometry Urinary tract infections
SM: Conception and design, acquisition of data, analysis and interpretation of data; SC: Writing of the manuscript. SM will act as guarantor for this paper.
Compliance with Ethical Standards
Conflict of Interest
- 5.Collins CH, Lyne PM. Microbiological methods. 5th ed. London: Butterworths; 1985. p. 128–34.Google Scholar
- 6.Bradley M, Schumann GB. Examination of urine. In: Henry JB, editor. Clinical diagnosis and management by laboratory methods. 17th ed. Philadelphia: WB Saunders; 1984. p. 380–458.Google Scholar
- 7.Manoni F, Fornasiero L, Ercolin M, et al. Quantificazione della batteriuria e dei leucociti urinari mediante Sysmex UF-1000i: confronto con la coltura quantitativa. Ital J Lab Med. 2009;5:44–9.Google Scholar
- 8.Manoni F, Ercolin M, Bountis I, et al. Valutazione dell’analizzatore Sysmex UF-1000i per l’esame automatizzato del sedimento urinario. Biochim Clin. 2009;33:550–5.Google Scholar
- 10.Hodson EM, Craig JC. Urinary tract infection in children. In: Avner ED, Harmon WE, Niaudet P, Yoshikawa F, Emma F, Goldstein SL, editors. Pediatric nephrology. 7th ed. Philadelphia: Lippincott Williams & Wilkins; 2016. p. 1696–714.Google Scholar
- 11.Evidence-based care guideline for medical management of first urinary tract infection in children 12 years of age or less. Cincinnati: UTI Guideline Team. Cincinnati Children’s Hospital Medical Center; 2006. p. 1–23.Google Scholar