Molecular Epidemiology of Rotavirus Strains in Under Five Children

Original Article
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Abstract

Objectives

To determine the prevalence and genotype distribution of rotaviruses in children presenting to authors’ hospital in Middle Black Sea region of Turkey. The results may supply important information about vaccine studies in Turkey.

Methods

Rotavirus antigen was detected by latex agglutination test and rotavirus RNA was detected by RT-PCR test. On the other hand, rotavirus positive samples were genotyped by semi-nested multiplex polymerase chain reaction.

Results

The highest rate of rotavirus positivity (46.9%) was observed among children in the 13 to 24 mo age group. All the positive-strains were in G1–4, G8–9, P [4], P [8], and P [9] genotypes. The most common G and P combination in present study was G9P[8] (n = 24, 28.9%).

Conclusions

The present results indicated that the most prevalent genotypes were G1, G9, P8, G9P[8] and G1P[8] in authors’ region. Rotavirus vaccines used in this region must include mainly these genotypes.

Keywords

Rotavirus Genotype Children Tokat Turkey 

Notes

Contributions

YB and GY: Provided the samples, did analysis and interpretation of the data; RD: designed the study and was responsible for genotyping. All the authors participated in writing and critical review of the manuscript. RD will act as guarantor for the paper.

Compliance with Ethical Statement

This study was approved by the ethics committee of the Ministry of Health, Dr. Abdurrahman Yurtaslan Oncology Training and Research Hospital, Ankara/Turkey (Protocol code: 2012–8/37). The verbal consent of parents of the child enrolled in this study was obtained prior to the sample collection. Because collection of fecal samples from children with suspected rotavirus infection being admitted to the hospital was a routine process for rotavirus diagnosis, verbal consent was approved by the ethics committees.

Conflict of Interest

None.

Source of Funding

None.

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Copyright information

© Dr. K C Chaudhuri Foundation 2017

Authors and Affiliations

  • Yunus Bulut
    • 1
  • Gülgün Yenişehirli
    • 1
  • Rıza Durmaz
    • 2
  1. 1.Department of Medical MicrobiologyGaziosmanpaşa University Faculty of MedicineTokatTurkey
  2. 2.Department of Medical MicrobiologyYıldırım Beyazıt University Faculty of MedicineAnkaraTurkey

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