Improving Quality of Banked Milk: Utility of Dornic Acid Test
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To identify, if Dornic acid test done on human milk bank sample is as effective as doing routine culture, both pre and post pasteurization.
The authors analyzed 477 samples, both pre and post pasteurization. Dornic acid measurement was performed by using N/9 NaOH and titrated to get dornicity of the sample. Senstivity, specificity, positive predictive value (PPV) and negative predictive value (NPV) at different Dornic acid levels were calculated. To find diagnostic power of this study, ROC curve was prepared. Dornic acid for paired pre and post pasteurization were noted and analysed for statistical significance.
A significant growth was seen in 21% (98/477) samples with coagulase negative staphylococci and gram negative organisms being the major contaminants seen prior to pasteurization. In the index study, Dornic acidity ≥4°D had a sensitivity of 98% but very low specificity of just over 10%. However, 8°D had 92% specificity and acceptable sensitivity of 72% in comparison to gold standard microbiological criteria, making it a good test for analyzing the quality of milk before pasteurization.
Dornic acid test at 8°D has a very high specificity with acceptable sensitivity in comparison to dornicity at 4°D. It can be used as a simple method to select better quality of milk sample prior to pasteurization.
KeywordsHuman milk bank Dornic acid Pasteurization
Dean, LTMMC and Hospital, Mumbai for permission to publish the research paper.
JM, SM: Conceived and designed the study; SG, DD: Involved in collection & analysis and interpreting the data; JM, SG: Drafting the manuscript. SwB: Contributed to laboratory sample analysis (calculating Dornic acidity), article write-up and statistical analysis; SuB: Contributed to microbiological analysis and final reporting of milk culture sample. All authors approved the final manuscript. JM will act as guarantor for this paper.
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- 2.World Health Organization. Infant and young child nutrition. Global strategy on infant and young child feeding. Report by the secretariat. Fifty-fifth world health assembly. A55/15. April 16, 2002.Google Scholar
- 5.Novak FR, Cordeiro DM. The correlation between aerobic mesophilic microorganism counts and dornic acidity in expressed human milk. J Pediatr. 2007;83:87–91.Google Scholar
- 7.Almeda D, Novak K, Sandoval B. Banco de Leite Humano: Funcionamiento, Prevençao e Controle de Riscos. Brasilia: Agência Nacional de Vigilância Sanitâria; 2007.Google Scholar
- 11.Escuder-Vieco D, Vázquez-Román S, Sánchez-Pallás J, Ureta-Velasco N, Mosqueda-Peña R, Pallás-alonso CR. Determination of acidity in donor milk: comparison of results obtained by 2 different analytical methods. J Hum Lact. 2016;32:NP73–5.Google Scholar