Childhood Brucellosis in Eastern India
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To investigate the presence of childhood brucellosis presenting as PUO (pyrexia of unknown origin) cases in Eastern zone of India.
Blood samples were collected from PUO patients aged ≤18 y. The main diagnostic tools were STAT, RBPT, ELISA- IgM, IgG and PCR. Although mainly PUO cases were selected for the study, other associated clinical manifestations were also noted.
The findings revealed significantly higher percentage of infection in female children (14.3%) than in male children (10.9%). The positive results by different diagnostic tools, STAT, RBPT, ELISA- IgM, ELISA-IgG and brucella genus specific PCR were 10.6%, 7.2%, 7.2%, 0.85% and 1.3% respectively. Main associated clinical symptoms were joint pain, low backache, fatigue and night sweat.
This hospital based study reflects a significant number of childhood brucellosis cases in Eastern zone of India, and thus emphasizes the need for further monitoring of such subjects.
KeywordsChildhood brucellosis PUO STAT RBPT ELISA PCR
The authors hereby acknowledge the support received from all staffs of Department of Microbiology, Peerless Hospital & B. K. Roy Research Centre, Kolkata.
All the authors were involved in the study. DD performed the study protocols, analyzed the data and drafted the manuscript. SD designed the study, critically reviewed, finalized the manuscript and will act as guarantor for this paper.
Compliance with Ethical Standards
Conflict of Interest
Source of Funding
This study was funded by the Department of Biotechnology (DBT), Government of India (Grant No. & Date: 102/IFD/SAN/3141/2012–2013 dated September, 27 2012).
- 1.Young EJ. Brucella species. In: Mandell GL, Benett JE, Dolin R, editors. Principles and Practice of Infectious Diseases. 5th ed. Philadelphia: Churchill Livingstone; 2000. p. 2386–93.Google Scholar
- 19.Araj GF, Kaufmann AF. Determination by enzyme linked immunosorbent assay of immunoglobulin G (IgG), IgM and IgA to Brucella melitensis major outer membrane proteins and whole-cell heat-killed antigens in sera of patients with brucellosis. J Clin Microbiol. 1989;27:1909–12.PubMedPubMedCentralGoogle Scholar
- 20.Pickering LK, Baker CJ, Kimberlin DW, Long SS. Report of the Committee on Infectious Diseases. Red Book. Elk Grove Village: American Academy of Pediatrics; 2009. p. 237–9.Google Scholar
- 21.Corbel MJ, Alton GG, Ariza J. editors. Brucellosis in humans and animals. Geneva: World Health Organization 2006.Google Scholar