The Indian Journal of Pediatrics

, Volume 85, Issue 4, pp 266–271 | Cite as

Childhood Brucellosis in Eastern India

  • Devarati Dutta
  • Anindita Sen
  • Debkishore Gupta
  • Prabir Kuila
  • Debasmita Chatterjee
  • Sugat Sanyal
  • Satadal DasEmail author
Original Article



To investigate the presence of childhood brucellosis presenting as PUO (pyrexia of unknown origin) cases in Eastern zone of India.


Blood samples were collected from PUO patients aged ≤18 y. The main diagnostic tools were STAT, RBPT, ELISA- IgM, IgG and PCR. Although mainly PUO cases were selected for the study, other associated clinical manifestations were also noted.


The findings revealed significantly higher percentage of infection in female children (14.3%) than in male children (10.9%). The positive results by different diagnostic tools, STAT, RBPT, ELISA- IgM, ELISA-IgG and brucella genus specific PCR were 10.6%, 7.2%, 7.2%, 0.85% and 1.3% respectively. Main associated clinical symptoms were joint pain, low backache, fatigue and night sweat.


This hospital based study reflects a significant number of childhood brucellosis cases in Eastern zone of India, and thus emphasizes the need for further monitoring of such subjects.


Childhood brucellosis PUO STAT RBPT ELISA PCR 



The authors hereby acknowledge the support received from all staffs of Department of Microbiology, Peerless Hospital & B. K. Roy Research Centre, Kolkata.


All the authors were involved in the study. DD performed the study protocols, analyzed the data and drafted the manuscript. SD designed the study, critically reviewed, finalized the manuscript and will act as guarantor for this paper.

Compliance with Ethical Standards

Conflict of Interest


Source of Funding

This study was funded by the Department of Biotechnology (DBT), Government of India (Grant No. & Date: 102/IFD/SAN/3141/2012–2013 dated September, 27 2012).


  1. 1.
    Young EJ. Brucella species. In: Mandell GL, Benett JE, Dolin R, editors. Principles and Practice of Infectious Diseases. 5th ed. Philadelphia: Churchill Livingstone; 2000. p. 2386–93.Google Scholar
  2. 2.
    Young EJ. An overview of human brucellosis. Clin Infect Dis. 1995;21:283–90.CrossRefPubMedGoogle Scholar
  3. 3.
    Pappas G, Akritidis N, Bosilkovski M, Tsianos E. Brucellosis. N Engl J Med. 2005;352:2325–36.CrossRefPubMedGoogle Scholar
  4. 4.
    Sharda DC, Lubani M. A study of brucellosis in childhood. Clin Pediatr (Phila). 1986;25:492–5.CrossRefGoogle Scholar
  5. 5.
    Gottesman G, Vanunu D, Maayan MC, et al. Childhood brucellosis in Israel. Pediatr Infect Dis J. 1996;15:610–5.CrossRefPubMedGoogle Scholar
  6. 6.
    Tanir G, Tufekci SB, Tuygun N. Presentation, complications and treatment outcome of brucellosis in Turkish children. Pediatr Int. 2009;51:114–9.CrossRefPubMedGoogle Scholar
  7. 7.
    Sutcu M, Gokceer D, Akturk H, et al. Brucellosis infection in a child with progressive familial intrahepatic cholestasis type 2 who had undergone liver transplantation. Pediatr Transplant. 2015;19:E146–8.CrossRefPubMedGoogle Scholar
  8. 8.
    Colmenero JD, Regnera JM, Martos F, et al. Complications associated with Brucella melitensis infection: a study of 530 cases. Medicine. 1996;75:195–211.CrossRefPubMedGoogle Scholar
  9. 9.
    Boschiroli ML, Foulongne V, O’Callaghan D. Brucellosis: a worldwide zoonosis. Curr Opin Microbiol. 2001;4:58–64.CrossRefPubMedGoogle Scholar
  10. 10.
    Schutze GE, Jacobs RF. Brucella. In: Kliegman RM, Stanton BF, St. Geme III JW, Schor NF, Behrman RE, editors. Nelson Textbook of Pediatrics. Philadelphia: Elsevier Saunders; 2011. p. 980–2.CrossRefGoogle Scholar
  11. 11.
    Mantur BG, Akki AS, Mangalgi SS, Patil SV, Gobbur RH, Peerapur BV. Childhood brucellosis- a microbiological, epidemiological and clinical study. J Trop Pediatr. 2004;50:153–7.CrossRefPubMedGoogle Scholar
  12. 12.
    Madkour MM. Childhood brucellosis. In: Madkour MM, editor. Brucellosis. Cambridge: Butterworths; 1989. p. 205–18.CrossRefGoogle Scholar
  13. 13.
    Feiz J, Sabbaghian H, Miralai M. Brucellosis due to B. melitensis in children. Clinical and epidemiologic observations on 95 patients studied in central Iran. Clin Pediatr (Phila). 1978;17:904–7.CrossRefGoogle Scholar
  14. 14.
    Havaldar PV, Kumar SY, Desai AS, Siddibhavi BM. Brucellosis in children. Indian Pediatr. 1987;24:995–8.PubMedGoogle Scholar
  15. 15.
    Mantur BG, Biradar MS, Bidri RC. Protean clinical manifestations and diagnostic challenges of human brucellosis in adults: 16 y experience in an endemic area. J Med Microbiol. 2006;55:897–903.CrossRefPubMedGoogle Scholar
  16. 16.
    Ciftdogan DY, Bayram N, Vardar F. Brucellosis as a cause of fever of unknown origin in children admitted to a tertiary hospital in the Aegean region of Turkey. Vector-Borne Zoonotic Dis. 2011;11:1037–40.CrossRefPubMedGoogle Scholar
  17. 17.
    Godfroid J, Cloeckaert A, Liantard JP, et al. From the discovery of the Malta fever’s agent to the discovery of a marine mammal reservoir, brucellosis has continuously been a re-emerging zoonosis. Vet Res. 2005;36:313–26.CrossRefPubMedGoogle Scholar
  18. 18.
    Solera J. Update on brucellosis: therapeutic challenges. Int J Antimicrob Agents. 2010;36:S18–20.CrossRefPubMedGoogle Scholar
  19. 19.
    Araj GF, Kaufmann AF. Determination by enzyme linked immunosorbent assay of immunoglobulin G (IgG), IgM and IgA to Brucella melitensis major outer membrane proteins and whole-cell heat-killed antigens in sera of patients with brucellosis. J Clin Microbiol. 1989;27:1909–12.PubMedPubMedCentralGoogle Scholar
  20. 20.
    Pickering LK, Baker CJ, Kimberlin DW, Long SS. Report of the Committee on Infectious Diseases. Red Book. Elk Grove Village: American Academy of Pediatrics; 2009. p. 237–9.Google Scholar
  21. 21.
    Corbel MJ, Alton GG, Ariza J. editors. Brucellosis in humans and animals. Geneva: World Health Organization 2006.Google Scholar
  22. 22.
    Ascoli A. Immunity and receptivity to Brucella abortus in the albino rat. Exp Med Surg. 1947;5:107–27.PubMedGoogle Scholar
  23. 23.
    Pugh LH. Effect of gonadal hormones on experimental infection of rats with Brucella abortus. Exp Biol Med. 1948;68:591–2.CrossRefGoogle Scholar
  24. 24.
    Memish Z. Brucellosis control in Saudi Arabia: prospects and challenges. J Chemother. 2001;13:11–7.CrossRefPubMedGoogle Scholar

Copyright information

© Dr. K C Chaudhuri Foundation 2017

Authors and Affiliations

  • Devarati Dutta
    • 1
  • Anindita Sen
    • 2
  • Debkishore Gupta
    • 3
  • Prabir Kuila
    • 1
  • Debasmita Chatterjee
    • 4
  • Sugat Sanyal
    • 5
  • Satadal Das
    • 1
    Email author
  1. 1.Brucella Research LaboratoryPeerless Hospital & B.K. Roy Research CentreKolkataIndia
  2. 2.Department of MicrobiologyMGM Medical CollegeKishanganjIndia
  3. 3.Department of Microbiology & Infection ControlCMRI and BM Birla Heart Research CentreKolkataIndia
  4. 4.Department of Food Technology & Biochemical EngineeringJadavpur UniversityKolkataIndia
  5. 5.Department of LaboratoryPeerless Hospital & B.K. Roy Research CentreKolkataIndia

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