Association of IL-10 Gene Polymorphism (−819C > T, −592C > A and −1082G > A) with Preterm Birth
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To analyze the association of IL-10 gene and its polymorphisms with preterm birth (PTB).
Five hundred and fifty nine women with term birth and 559 with preterm birth were recruited from Lucknow, India. Genetic association analysis was conducted between cases and controls. Subjects recruited as cases were women (aged between 18–40 y) with singleton delivery before 37 wk of gestation and controls were with delivery after or on 37 wk. The genotyping was performed for rs1800871, rs1800872 and rs1800896 for assessing the allelic distribution, haplotypic association and linkage disequilibrium analysis. IL-10mRNA levels were evaluated by real time quantitative polymerase chain reaction (PCR) method.
The risk of PTB was found significant in women carrying IL-10 (−1082) GA genotype [OR=1.72(1.7–2.5), p=0.006]. The haplotypic analysis of studied polymorphisms for rs1800871, rs1800872 and rs1800896 depicted the association of ATA (p=0.02) and ATC (p=0.01) haplotypes with PTB. The IL-10 mRNA levels were significantly lower in cases (p=0.05).
IL-10 marks a protective impact in the inflammatory pathway of PTB.
KeywordsCytokines Inflammation Interleukin Gene expression Haplotype Polymorphisms Preterm birth
MP, SA: Conception or design of the experimental work and data collection; MP, SA, US, AAM: Acquisition, analysis, and interpretation of data. SA will act as guarantor for the paper.
Compliance with Ethical Standards
Conflict of Interest
Source of Funding
The study was financially supported by Indian Council of Medical Research (ICMR) 5/7/513/11-RHN.
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