Skip to main content
SpringerLink
Log in

Does Mesenchymal Stem Cell Population in Umbilical Cord Blood Vary at Different Gestational Periods?

  • Original Article
  • Published:
The Indian Journal of Pediatrics Aims and scope Submit manuscript

Abstract

Objective

To identify and quantitatively determine Mesenchymal stem cells (MSCs) in the umbilical cord blood (UCB) of neonates born at different gestational periods.

Methods

UCB was collected at birth in neonates of three different gestational groups. The mononuclear cells (MNCs) were phenotypically analyzed by flow cytometer.

Results

The yield of total MNCs did not differ much with gestation; the average values were 22.6 ± 6.48 × 106 cells/ml. The MSCs were significantly higher in the lower gestation group. These were 0.0219 ± 0.012 %, 0.0044 ± 0.003 % and 0.0022 ± 0.003 % in 28 to 31 wk, 32 to 35 wk and >36 wk, respectively (P = 0.00). There was a significant inverse correlation between the gestational age and the presence of MSCs with a correlation co-efficient of −0.54 (P = 0.0001).

Conclusions

The MSCs population was significantly higher in infants born at lesser gestation than those born at term gestation.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2

Similar content being viewed by others

References

  1. Sanz GF, Saavedra S, Jiménez C, et al. Unrelated donor cord blood transplantation in adults with chronic myelogenous leukemia: results in nine patients from a single institution. Bone Marrow Transplant. 2001;27:693–701.

    Article  PubMed  CAS  Google Scholar 

  2. D’ippolipo G, Schiller PC, Ricordi C, Roos BA, Howard GA. Age related ostiogenic potential of mesenchymal stromal stem cells from human vertebral bone marrow. J Bone Miner Res. 1999;14:1115–22.

    Article  Google Scholar 

  3. Lee OK, Kuo TK, Chen WM, Lee KD, Hsieh SL, Chen TH. Isolation of multipotent mesenchymal stem cells from umbilical cord blood. Blood. 2004;103:1669–75.

    Article  PubMed  CAS  Google Scholar 

  4. Kim JW, Kim SY, Park SY, et al. Mesenchymal progenitor cells in the human umbilical cord. Ann Hematol. 2004;83:733–8.

    Article  PubMed  CAS  Google Scholar 

  5. Gang EJ, Hong SH, Jeong JA, et al. In vitro mesengenic potential of human umbilical cord blood derived mesenchymal stem cells. Biochem Biophys Res Commun. 2004;321:102–8.

    Article  PubMed  CAS  Google Scholar 

  6. Luo F, Yang YJ, Wang X, et al. Factors influencing successful isolation of mesenchymal stem cells from human cord blood. Zhonghua Er Ke Za Zhi. 2006;44:509–12.

    PubMed  Google Scholar 

  7. Erices A, Conget P, Minguell JJ, et al. Mesenchymal progenitor cells in human umbilical cord blood. Br J Haematol. 2000;109:235–42.

    Article  PubMed  CAS  Google Scholar 

  8. Ballard JL, Khoury JC, Wedig K, Wang L, Eilers-Walsman BL, Lipp R. New Ballard Score, expanded to include extremely premature infants. J Pediatr. 1991;119:417–23.

    Article  PubMed  CAS  Google Scholar 

  9. Lian Q, Lye E, Suan Yeo K, et al. Derivation of clinically compliant MSCs from CD105+, CD24- differentiated human ESCs. Stem Cells. 2007;25:425–36.

    Article  PubMed  CAS  Google Scholar 

  10. Dvorakova J, Hruba A, Velebny V, Kubala L. Isolation and characterization of mesenchymal stem cell population entrapped in bone marrow collection sets. Cell Biol Int. 2008;32:1116–25.

    Article  PubMed  CAS  Google Scholar 

  11. Lu LL, Liu YJ, Yang SG, et al. Isolation and characterization of human umbilical cord mesenchymal stem cells with hematopoiesis-supportive function and other potentials. Haematologica. 2006;91:1017–26.

    PubMed  CAS  Google Scholar 

  12. Li CD, Zhang WY, Li HL, et al. Isolation and identification of a multilineage potential mesenchymal cell from human placenta. Placenta. 2005 Sep 17[Epub ahead of print].

  13. Hu Y, Liao L, Wang Q, et al. Isolation and identification of mesenchymal stem cells from human fetal pancreas. J Lab Clin Med. 2003;141:342–9.

    Article  PubMed  CAS  Google Scholar 

  14. Kern S, Eichler H, Stoeve J, Klüter H, Bieback K. Comparative analysis of mesenchymal stem cells from bone marrow, umbilical cord blood, or adipose tissue. Stem Cells. 2006;24:1294–301.

    Article  PubMed  CAS  Google Scholar 

  15. Clapp DW, Baley JE, Gerson SL. Gestational age-dependent changes in circulating, hematopoietic stem cells in newborn infants. J Lab Clin Med. 1989;113:422–7.

    PubMed  CAS  Google Scholar 

  16. Forestier F, Daffos F, Catherine N, Renard M, Andreux JP. Developmental hematopoiesis in normal human fetal blood. Blood. 1991;77:2360–3.

    PubMed  CAS  Google Scholar 

  17. Shields LE, Andrews RG. Gestational age changes in circulating CD34+ hematopoietic stem/progenitor cells in fetal cord blood. Am J Obstet Gynecol. 1998;178:931–7.

    Article  PubMed  CAS  Google Scholar 

Download references

Acknowledgements

Consumables for this study was made available from the research grant provided by the All India Institute of Medical Sciences, New Delhi. Authors are grateful to Stem cell Biology lab, National Institute of Immunology, India for flow cytometric analysis of the samples.

Conflict of Interest

None.

Role of funding Source

The study was supported by the funds from the AIIMS Research Fund.

Author information

Authors and Affiliations

  1. Department of Pediatrics, Division of Neonatology, All India Institute of Medical Sciences, Ansari Nagar, New Delhi, 110029, India

    Ashish Jain, M. Jeevashankar, Ramesh Agarwal, A. K Deorari & V. K Paul

  2. Department of Medical Oncology, All India Institute of Medical Sciences, Ansari Nagar, New Delhi, India

    Nitin Mathur

  3. Stem Cell Biology Laboratory, National Institute of Immunology, New Delhi, India

    Asok Mukhopadhyay

Authors
  1. Ashish Jain
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Nitin Mathur
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. M. Jeevashankar
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Asok Mukhopadhyay
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Ramesh Agarwal
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. A. K Deorari
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. V. K Paul
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Ramesh Agarwal.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Jain, A., Mathur, N., Jeevashankar, M. et al. Does Mesenchymal Stem Cell Population in Umbilical Cord Blood Vary at Different Gestational Periods?. Indian J Pediatr 80, 375–379 (2013). https://doi.org/10.1007/s12098-012-0844-3

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s12098-012-0844-3

Keywords

Search

Navigation