Skip to main content

Advertisement

SpringerLink
Log in

Hsa_circ_0102899 promotes epithelial–mesenchymal transition in non-small cell lung cancer

  • RESEARCH ARTICLE
  • Published:
Clinical and Translational Oncology Aims and scope Submit manuscript

Abstract

Objective

The significance of circular RNAs (circRNAs) has been identified in the progression of non-small cell lung cancer (NSCLC). Consistently, our study probed the functional actions of hsa_circ_0102899 (circ_0102899) in NSCLC cells.

Methods

circ_0102899 expression was checked in NSCLC tissues, as well as its correlation with clinical characteristics of patients, Using A459 cells, transfection to alter gene expression was performed, thus measuring the changes of proliferation, apoptosis, migration, and apoptosis, as well as epithelial–mesenchymal transition (EMT)-related proteins. circ_0102899’s effects in vivo were validated by tumor xenograft assay. Finally, the regulatory mechanism of circ_0102899 was investigated.

Results

circ_0102899 indicated a high-expression level in NSCLC tissues which was associated with NSCLC tumor characteristics. Functionally, circ_0102899 knockdown not only inhibited the growth and EMT process of NSCLC cells, but also inhibited tumor formation in vivo. In terms of the regulatory mechanism, circ_0102899 had a binding to miR-885-5p to target eukaryotic translation initiation factor 4γ2 (EIF4G2). circ_0102899 mediated miR-885–5/EIF4G2 axis to accelerate the process of cell malignant behavior in NSCLC.

Conclusion

circ_0102899 promotes EMT and metastasis in NSCLC by regulating the miR-885-5p/EIF4G2 axis.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig.1
Fig. 2
Fig. 3
Fig. 4
Fig. 5
Fig. 6
Fig. 7

Similar content being viewed by others

Data availability

Not applicable.

References

  1. de Groot PM, Wu CC, Carter BW, Munden RF. The epidemiology of lung cancer. Transl Lung Cancer Res. 2018;7(3):220–33.

    Article  PubMed  PubMed Central  Google Scholar 

  2. Bray F, Ferlay J, Soerjomataram I, Siegel RL, Torre LA, Jemal A. Global cancer statistics 2018: GLOBOCAN estimates of incidence and mortality worldwide for 36 cancers in 185 countries. CA Cancer J Clin. 2018;68(6):394–424.

    Article  PubMed  Google Scholar 

  3. Zeng X, Liu D, Peng G, Liu J, Yang H. MiroRNA-31-3p promotes the invasion and metastasis of non-small-cell lung cancer cells by targeting forkhead box 1 (FOXO1). Comput Math Methods Med. 2022;2022:4597087.

    Article  PubMed  PubMed Central  Google Scholar 

  4. Blandin Knight S, Crosbie PA, Balata H, Chudziak J, Hussell T, Dive C. Progress and prospects of early detection in lung cancer. Open Biol. 2017;7(9):170070.

    Article  PubMed  PubMed Central  Google Scholar 

  5. Dong K, Liu L, Yu Z, Wu D, Zhang Q, Huang X, et al. Brain metastases from lung cancer with neuropsychiatric symptoms as the first symptoms. Transl Lung Cancer Res. 2019;8(5):682–91.

    Article  PubMed  PubMed Central  Google Scholar 

  6. Niu H, Qu A, Guan C. Suppression of MGAT3 expression and the epithelial-mesenchymal transition of lung cancer cells by miR-188-5p. Biomed J. 2021;44(6):678–85.

    Article  CAS  PubMed  Google Scholar 

  7. Ebbesen KK, Hansen TB, Kjems J. Insights into circular RNA biology. RNA Biol. 2017;14(8):1035–45.

    Article  PubMed  Google Scholar 

  8. Caba L, Florea L, Gug C, Dimitriu DC, Gorduza EV. Circular RNA-Is the Circle Perfect? Biomolecules. 2021;11(12):1755.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  9. Zhu Y, Ma C, Lv A, Kou C. Circular RNA circ_0010235 sponges miR-338-3p to play oncogenic role in proliferation, migration and invasion of non-small-cell lung cancer cells through modulating KIF2A. Ann Med. 2021;53(1):693–706.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  10. Tan Q, Liu C, Shen Y, Huang T. Circular RNA circ_0000517 facilitates the growth and metastasis of non-small cell lung cancer by sponging miR-326/miR-330-5p. Cell J. 2021;23(5):552–61.

    PubMed  PubMed Central  Google Scholar 

  11. Huang Y, Qin S, Gu X, Zheng M, Zhang Q, Liu Y, et al. Comprehensive assessment of serum hsa_circ_0070354 as a novel diagnostic and predictive biomarker in non-small cell lung cancer. Front Genet. 2021;12: 796776.

    Article  CAS  PubMed  Google Scholar 

  12. Aghanoori MR, Mirzaei B, Tavallaei M. MiRNA molecular profiles in human medical conditions: connecting lung cancer and lung development phenomena. Asian Pac J Cancer Prev. 2014;15(22):9557–65.

    Article  PubMed  Google Scholar 

  13. Su WZ, Ren LF. MiRNA-199 inhibits malignant progression of lung cancer through mediating RGS17. Eur Rev Med Pharmacol Sci. 2019;23(8):3390–400.

    PubMed  Google Scholar 

  14. Zhang M, Shi H, Zhang C, Zhang SQ. MiRNA-621 inhibits the malignant progression of non-small cell lung cancer via targeting SIX4. Eur Rev Med Pharmacol Sci. 2019;23(11):4807–14.

    CAS  PubMed  Google Scholar 

  15. Jiang Z, Cui H, Zeng S, Li L. miR-885-5p inhibits invasion and metastasis in gastric cancer by targeting malic enzyme 1. DNA Cell Biol. 2021;40(5):694–705.

    Article  CAS  PubMed  Google Scholar 

  16. Shou J, Gao H, Cheng S, Wang B, Guan H. LncRNA HOXA-AS2 promotes glioblastoma carcinogenesis by targeting miR-885-5p/RBBP4 axis. Cancer Cell Int. 2021;21(1):39.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  17. Li C, Wang X, Song Q. MicroRNA 885–5p inhibits hepatocellular carcinoma metastasis by repressing AEG1. Onco Targets Ther. 2020;13:981–8.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  18. Li S, Shao J, Lou G, Wu C, Liu Y, Zheng M. MiR-144-3p-mediated dysregulation of EIF4G2 contributes to the development of hepatocellular carcinoma through the ERK pathway. J Exp Clin Cancer Res. 2021;40(1):53.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  19. Zhang Z, Cui Z, Xie Z, Li C, Xu C, Guo X, et al. Deubiquitinase USP5 promotes non-small cell lung cancer cell proliferation by stabilizing cyclin D1. Transl Lung Cancer Res. 2021;10(10):3995–4011.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  20. Ning Y, Zheng H, Zhan Y, Liu S, Yang Y, Zang H, et al. Overexpression of P4HA1 associates with poor prognosis and promotes cell proliferation and metastasis of lung adenocarcinoma. J Cancer. 2021;12(22):6685–94.

    Article  PubMed  PubMed Central  Google Scholar 

  21. Wu C, Lin W, Fu F. Long non-coding RNA DLX6-AS1 knockdown suppresses the tumorigenesis and progression of non-small cell lung cancer through microRNA-16-5p/BMI1 axis. Transl Cancer Res. 2021;10(8):3772–87.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  22. Ma J, Li Q, Li Y. CircRNA PRH1-PRR4 stimulates RAB3D to regulate the malignant progression of NSCLC by sponging miR-877-5p. Thorac Cancer. 2022;13(5):690–701.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  23. Chen F, Li M, Wang L. LncRNA CASC11 promotes hepatocellular carcinoma progression via upregulation of UBE2T in a m(6)A-dependent manner. Front Oncol. 2021;11: 772671.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  24. Li B, Lou G, Zhang J, Cao N, Yu X. Repression of lncRNA PART1 attenuates ovarian cancer cell viability, migration and invasion through the miR-503-5p/FOXK1 axis. BMC Cancer. 2022;22(1):124.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  25. Li W, Yang P, Zhong C, Shen X, Shi X, Li X. The circ-PITX1 promotes non-small cell lung cancer development via the miR-30e-5p/ITGA6 axis. Cell Cycle. 2022;21(3):304–21.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  26. Ma Z, Chen G, Chen Y, Guo Z, Chai H, Tang Y, et al. MiR-937-3p promotes metastasis and angiogenesis and is activated by MYC in lung adenocarcinoma. Cancer Cell Int. 2022;22(1):31.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  27. Yang W, Yin Y, Bi L, Wang Y, Yao J, Xu L, et al. MiR-182-5p promotes the metastasis and epithelial-mesenchymal transition in non-small Cell lung cancer by targeting EPAS1. J Cancer. 2021;12(23):7120–9.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  28. Wei H, Li L, Zhang H, Xu F, Chen L, Che G, et al. Circ-FOXM1 knockdown suppresses non-small cell lung cancer development by regulating the miR-149-5p/ATG5 axis. Cell Cycle. 2021;20(2):166–78.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  29. Jeck WR, Sorrentino JA, Wang K, Slevin MK, Burd CE, Liu J, et al. Circular RNAs are abundant, conserved, and associated with ALU repeats. RNA. 2013;19(2):141–57.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  30. Zhao ZJ, Shen J. Circular RNA participates in the carcinogenesis and the malignant behavior of cancer. RNA Biol. 2017;14(5):514–21.

    Article  PubMed  Google Scholar 

  31. Liu Y, Ao X, Yu W, Zhang Y, Wang J. Biogenesis, functions, and clinical implications of circular RNAs in non-small cell lung cancer. Mol Ther Nucleic Acids. 2022;27:50–72.

    Article  PubMed  Google Scholar 

  32. Yang J, Hao R, Zhang Y, Deng H, Teng W, Wang Z. Construction of circRNA-miRNA-mRNA network and identification of novel potential biomarkers for non-small cell lung cancer. Cancer Cell Int. 2021;21(1):611.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  33. Jiang SP, Li ZR. MiR-503-5p regulates cell epithelial-to-mesenchymal transition, metastasis and prognosis of hepatocellular carcinoma through inhibiting WEE1. Eur Rev Med Pharmacol Sci. 2019;23(5):2028–37.

    PubMed  Google Scholar 

  34. Chai Y, Xie M. LINC01579 promotes cell proliferation by acting as a ceRNA of miR-139-5p to upregulate EIF4G2 expression in glioblastoma. J Cell Physiol. 2019;234(12):23658–66.

    Article  CAS  PubMed  Google Scholar 

  35. Tong J, Lu J, Mao X, Zhu Z, Wang Y, Lou M, et al. Circular RNA-UBE2D2 accelerates the proliferation and metastasis of non-small cell lung cancer cells via modulating microRNA-376a-3p/eukaryotic translation initiation factor 4γ2 axis. Bioengineered. 2022;13(3):5942–53.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  36. Hao GJ, Hao HJ, Ding YH, Wen H, Li XF, Wang QR, et al. Suppression of EIF4G2 by miR-379 potentiates the cisplatin chemosensitivity in nonsmall cell lung cancer cells. FEBS Lett. 2017;591(4):636–45.

    Article  CAS  PubMed  Google Scholar 

Download references

Acknowledgements

Not applicable.

Funding

The Project of Kunming Municipal Commission of Health and Family Planning [2018-SW (province)-003]: the value of blood、BALF、urine GM test in early diagnosis of non-granular IPA patients (Kunming Municipal Health Commission: 2020-03-02-112).

Author information

Authors and Affiliations

  1. Department of Thoracic Surgery, Yan’an Affiliated Hospital of Kunming Medical University, No. 245, East Renmin Road, Panlong District, Kunming City, 650051, Yunnan Province, China

    YanFei Wang & DaLin Xiong

  2. Department of Respiratory and Critical Care Medicine, Yan’an Affiliated Hospital of Kunming Medical University, No. 245, East Renmin Road, Panlong District, Kunming City, 650051, Yunnan Province, China

    JieMing He & YongXue Liang

Authors
  1. YanFei Wang
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. JieMing He
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. YongXue Liang
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. DaLin Xiong
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding authors

Correspondence to YongXue Liang or DaLin Xiong.

Ethics declarations

Conflict of interest

The authors have no conflicts of interest to declare.

Ethics approval and consent to participate

The present study was approved by the Ethics Committee of Yan'an Af ffiliated Hospital of Kunming Medical University and written informed consent was provided by all patients prior to the study start All procedures were performed in accordance with the ethical standards of the Institutional Review Board and the Declaration of Helsinki, and its later parameters or comparable ethical standards(No. YA20197166).

Informed consent

Informed consent was observed from all individual participants included in the study.

Additional information

Publisher's Note

Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.

Supplementary Information

Below is the link to the electronic supplementary material.

12094_2023_3220_MOESM1_ESM.tif

Supplementary Figure 1 Down-regulation of circ_0102899 can inhibit the proliferation, migration, invasion and EMT process of H1299 cells, and promote cell apoptosis. A. The transfection efficiency of sh-circ_0102899 in H1299 cells. B-E. Proliferation, apoptosis, migration and invasion ability of H1299 cells.F. EMT-related protein expression levels in H1299 cells. Measurement data are expressed as mean ± standard deviation; * P < 0.05, N = 3.Supplementary file1 (TIF 6210 kb)

Rights and permissions

Springer Nature or its licensor (e.g. a society or other partner) holds exclusive rights to this article under a publishing agreement with the author(s) or other rightsholder(s); author self-archiving of the accepted manuscript version of this article is solely governed by the terms of such publishing agreement and applicable law.

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Wang, Y., He, J., Liang, Y. et al. Hsa_circ_0102899 promotes epithelial–mesenchymal transition in non-small cell lung cancer. Clin Transl Oncol 25, 3252–3262 (2023). https://doi.org/10.1007/s12094-023-03220-7

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s12094-023-03220-7

Keywords

Search

Navigation