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Long noncoding RNA HOTAIR promotes breast cancer development through the lncRNA HOTAIR/miR-1/GOLPH3 axis

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A Correction to this article was published on 02 June 2023

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Abstract

Background

The lncRNA HOTAIR is frequently overexpressed in breast cancer tissues and plays an important role in the development of breast cancer. Here, we investigated the effect of the lncRNA HOTAIR on the biological behaviour of breast cancer cells and its molecular mechanism.

Methods

We investigated the level of HOTAIR in breast cancer and its clinical pathological characteristics by bioinformatic methods. Then, we evaluated the effects of HOTAIR and miRNA-1 expression on the biological behaviour of breast cancer cells by qPCR, Cell Counting Kit-8 (CCK-8) assay, clonogenic assays, Transwell assay and flow cytometry for cell proliferation, invasion migration and apoptosis, and cell cycle analysis. Finally, the target genes of the lncRNA HOTAIR/miR-1/GOLPH3 regulatory axis were validated by luciferase reports.

Results

The expression of HOTAIR in breast cancer tissues was significantly higher than that in normal breast tissues (P < 0.05). Silencing of HOTAIR suppressed cell proliferation, invasion and migration, promoted apoptosis and induced G1 phase block in breast cancer (P < 0.0001). We also verified that miR-1 is a target of HOTAIR and that GOLPH3 is a target of miR-1 by luciferase reporter assays (P < 0.001).

Conclusions

The expression of HOTAIR was significantly elevated in breast cancer tissues. Reducing the expression of HOTAIR inhibited the proliferation, invasion and migration of breast cancer cells and promoted apoptosis, and the mechanism was mainly the effect of the lncRNA HOTAIR/miR-1/GOLPH3 regulatory axis on the biological behaviour of breast cancer cells.

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Data availability

Data availability statement is available for this paper at https://doi.org/10.6084/m9.figshare.21506364.

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Funding

The present study was supported by the National Natural Science Foundation of China (grant no. 81960542 and 81960517), Science and Technology Project of Yunnan Provincial Science and Technology Department (grant no. 202001AU070053, 202001AU070093 and 202201AY070001-169), Yunnan Health Training Project of High Level Talents (grant no. H-2019075) and Beijing Science and Technology Innovation Medical Development Foundation (grant no. KC2021-JK-0044-6).

Author information

Author notes

  1. Jiawen Zhao, Lei Zhang, Yingzhu Zhao, and Nan Wu have contributed equally to this work.

Authors and Affiliations

  1. Department of Breast Surgery, The Third Affiliated Hospital of Kunming Medical University, Yunnan Cancer Hospital, Kunming, China

    Jiawen Zhao, Lei Zhang, Rong Guo, Chunxiang Li, Kai Zheng, Dequan Liu & Shicong Tang

  2. Department of Breast and Thyroid Surgery, The People’s Hospital of Guangxi Zhuang Autonomous Region, Nanning, China

    Yingzhu Zhao

  3. Department of Medical School, Yunnan College of Business Management, Kunming, China

    Nan Wu

  4. Department of Clinical Laboratory, Yunnan Cancer Hospital, Kunming, China

    Xi Zhang

  5. Second Department of General Surgery, Southern Central Hospital of Yunnan Province, The First People’s Hospital of Honghe State, Honghe Hospital Affiliated to Kunming Medical University, Mengzi, Honghe, China

    Huimeng Li

Authors
  1. Jiawen Zhao
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Contributions

LZ and JWZ contributed to the experiments and clinical data interpretation. JWZ and SCT discussed the results and analysed the data and wrote the manuscript conceived and designed the experiments. YZZ, NW and XZ contributed to the statistical analysis. RG, HML and CXL contributed to polishing the English to improve the quality of this manuscript. SCT, KZ and DQL supervised and directed the overall project. All authors read and approved the final manuscript.

Corresponding authors

Correspondence to Kai Zheng, Dequan Liu or Shicong Tang.

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Zhao, J., Zhang, L., Zhao, Y. et al. Long noncoding RNA HOTAIR promotes breast cancer development through the lncRNA HOTAIR/miR-1/GOLPH3 axis. Clin Transl Oncol 25, 3420–3430 (2023). https://doi.org/10.1007/s12094-023-03197-3

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