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Circular RNA circHIPK3 modulates prostate cancer progression via targeting miR-448/MTDH signaling

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Abstract

Purpose

Prostate cancer (PCa) is one of the most diagnosed cancers in men worldwide. Several studies have identified that circular RNAs (circRNAs) have a crucial impact on the biological processes in PCa. Therefore, it is necessary to study the molecular mechanism of circRNAs in tumor progression and metastasis.

Methods

RNA interference was used to decrease circHIPK3 and MTDH expression. Overexpression vector was used to increase circHIPK3 and MTDH expression. Luciferase reporter assay were used to detect the relationship between circHIPK3 and miR-448 or between miR-448 and MTDH. MTT assay, colony formation assay and transwell assay were used to measure proliferation and migration of PCa cells.

Results

Circular RNA circHIPK3 was significantly increased in PCa tissues and cell lines. And overexpression of circHIPK3 promoted the migration, proliferation, and invasion of PC-3 and 22Rv1 cells, while knockdown of circHIPK3 markedly repressed the above-mentioned series of biological processes. Furthermore, circHIPK3 promoted metadherin (MTDH) expression by sponging miR‐448. In vivo experiments, it was also found that overexpression of circHIPK3 significantly promoted tumor growth.

Conclusions

Our research shows that circHIPK3 plays a carcinogenic effect in PCa by regulating the miR-448/MTDH axis, indicating that circHIPK3 may be a potential therapeutic target for PCa.

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Acknowledgements

This study was supported by the Natural Science Foundation of Jiangsu Province (BK20151158); Innovation Team Project of Xuzhou Central Hospital ( XZB201607, XZB201610); Youth Medical Key Talent Project of Jiangsu Province (QNRC2016389, QNRC2016386); 333 Key Research and Development Plan Project of Jiangsu Province (BRA2017294, BE217635).

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Author notes

  1. D.C. Liu and L.L. Song contributed equally to this work.

Authors and Affiliations

  1. Department of Urology, Xuzhou Central Hospital, No. 199 Jiefang South Road, Xuzhou, 221009, Jiangsu, People’s Republic of China

    D. C. Liu,  X. Z. Li, Q. Liang,  Z. G. Zhang &  C. H. Han

  2. Department of Respiratory, Xuzhou Central Hospital, Xuzhou, 221009, People’s Republic of China

    L. L. Song

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  1. D. C. Liu
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  2. L. L. Song
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  5. Z. G. Zhang
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  6. C. H. Han
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Contributions

DL and LS are responsible for the conception or design of the work. XL and QL contribute the acquisition, analysis, or interpretation of data for the work. ZZ and CH conduct cell viability assay and luciferase reporter assay. All authors finally approved the manuscript version to be published.

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Correspondence to C. H. Han.

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The authors declare that they have no conflict of interest.

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The study was approved by Ethical Committee of Xuzhou Central Hospital and conducted in accordance with the ethical standards.

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Liu, .C., Song, .L., Li, .Z. et al. Circular RNA circHIPK3 modulates prostate cancer progression via targeting miR-448/MTDH signaling. Clin Transl Oncol 23, 2497–2506 (2021). https://doi.org/10.1007/s12094-021-02650-5

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  • DOI: https://doi.org/10.1007/s12094-021-02650-5

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