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LncRNA DCST1-AS1 downregulates miR-29b through methylation in glioblastoma (GBM) to promote cancer cell proliferation

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Abstract

Introduction

The role of DCST1-AS1 has been investigated in several types of cancer, while the role of DCST1-AS1 in glioblastoma (GBM) is unclear. This study aimed to investigate the role of DCST1-AS1 in GBM.

Methods

GBM and paired non-tumor tissues were collected from 62 GBM patients. Expression levels of DCST1-AS1 and miR-29b in paired tissue samples were determined by RT-qPCR. The role of DCST1-AS1 in regulating the methylation of miR-29b was assessed by methylation-specific PCR (MSP). Cell proliferation was analyzed by cell proliferation assay.

Results

It was observed that the upregulation of DCST1-AS1 in GBM predicted poor survival. MiR-29b was downregulated in GBM and inversely correlated with the expression of DCST1-AS1. In GBM cells, overexpression of DCST1-AS1 resulted in the downregulation of miR-29b and the increased methylation level of miR-29b gene. Overexpression of DCST1-AS1 resulted in increased cell proliferation. Moreover, Overexpression of DCST1-AS1 significantly reversed the inhibitory effects of miR-29b on cancer cell proliferation.

Conclusion

DCST1-AS1 may downregulate miR-29b through methylation in GBM to promote cancer cell proliferation.

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Funding

The work was supported by the Department of Rehabilitation, Huangshi Central Hospital, Affiliated Hospital of Hubei Polytechnic University.

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Authors and Affiliations

  1. Department of NeurosurgeryHuangshi Central Hospital, Affiliated Hospital of Hubei Polytechnic University, Huangshi City, 435000, Hubei Province, People’s Republic of China

    S. Hu, Y. Yao & X. Hu

  2. Department of Rehabilitation, Huangshi Central Hospital, Affiliated Hospital of Hubei Polytechnic University, No. 141 Tianjin Road, Huangshi Gang District, Huangshi City, 435000, Hubei Province, People’s Republic of China

    Y. Zhu

Authors
  1. S. Hu
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  2. Y. Yao
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  3. X. Hu
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  4. Y. Zhu
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Contributions

YZ supervised the whole study, data analysis, manuscript preparation; SH, YY and XH had data collection and analysis, manuscript preparation.

Corresponding author

Correspondence to Y. Zhu.

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Conflict of interest

The authors declare that they have no competing interests.

Ethical approval

Ethical approval was obtained from the Ethics Committee of the Second Hospital of Shandong University. All procedures performed in studies involving human participants were in accordance with the 1964 Helsinki declaration and its later amendments or comparable ethical standards. All patients signed the informed consent.

Informed consent

Informed consent was obtained from all individual participants included in the study.

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Hu, S., Yao, Y., Hu, X. et al. LncRNA DCST1-AS1 downregulates miR-29b through methylation in glioblastoma (GBM) to promote cancer cell proliferation. Clin Transl Oncol 22, 2230–2235 (2020). https://doi.org/10.1007/s12094-020-02363-1

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  • DOI: https://doi.org/10.1007/s12094-020-02363-1

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