Clinical and Translational Oncology

, Volume 19, Issue 6, pp 695–703

miR-26a-5p suppresses tumor metastasis by regulating EMT and is associated with prognosis in HCC

Research Article

DOI: 10.1007/s12094-016-1582-1

Cite this article as:
Chang, L., Li, K. & Guo, T. Clin Transl Oncol (2017) 19: 695. doi:10.1007/s12094-016-1582-1
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Abstract

Objective

To determine the role of miR-26a-5p in tumor invasion and metastasis in hepatocellular carcinoma (HCC).

Methods

We evaluated miR-26a-5p expression in HCC tissues by quantitative PCR and then analyzed its clinical significance using a Cox regression model. Transwell and nude mouse models were used to examine tumor metastasis in vitro and in vivo, respectively. The relationship between miR-26a-5p and epithelial-mesenchymal transition was also investigated by q-PCR and western blot.

Results

Strong downregulation of miR-26a-5p was observed in tumor tissues compared to paired adjacent normal tissues. Moreover, patients with low miR-26a-5p expression had a significantly poorer prognosis than those with high expression. The multivariate analysis indicated that miR-26a-5p expression was an independent prognostic indicator. The experimental transwell model and athymic mouse model revealed that miR-26a-5p depressed tumor metastasis in vitro and in vivo, respectively. In addition, the decreased miR-26a-5p level observed in HCC was associated with reduced E-cadherin expression and upregulation of vimentin, which affects the molecular mechanism of EMT.

Conclusion

Downregulation of miR-26a-5p promotes tumor metastasis by targeting EMT and influences the prognosis of HCC patients. Therefore, miR-26a-5p has potential as a new biomarker and therapeutic target.

Keywords

miR-26a-5p Tumor metastasis EMT Prognosis 

Copyright information

© Federación de Sociedades Españolas de Oncología (FESEO) 2016

Authors and Affiliations

  1. 1.Department of General SurgeryZhongnan Hospital of Wuhan UniversityWuhanPeople’s Republic of China

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