Clinical and Translational Oncology

, Volume 16, Issue 3, pp 293–300 | Cite as

HIV-1 viral protein R downregulates Ebp1 and stabilizes p53 in glioblastoma U87MG cells

  • S. Zhang
  • B. ZhangEmail author
  • X. Xu
  • L. Wang
  • X. Feng
  • Q. Wang
  • H. Huang
  • J. Wu
  • P. Li
  • J. WangEmail author
Research Article



HIV-1 viral protein R (Vpr) inhibits cell growth and induces apoptosis in a wide range of cancers. However, the mechanism by which Vpr induces cell cycle arrest and apoptosis in GBM cell lines is unclear. The present work was taken to detect the proteins interacted with Vpr in U87MG cells.


We analyzed the differential expression of proteins between glioblastoma cell U87MG treated with Ad-Vpr and untreated by 2-DE. We used antibody array analysis to analyze the common molecules in the apoptosis of U87MG induced by Vpr.


We analyzed the differential expression of proteins between U87MG cell treated with Ad-Vpr and untreated, and found that proteins related to DNA damage repair or different apoptosis pathways were involved in the G2 arrest and apoptosis mediated by Vpr. In addition, proliferation-associated protein 2G4 (PA2G4), also known as Ebp1, was down-regulated and p53 was up-regulated in U87MG cells treated with Ad-Vpr.


Our data suggest that Vpr may inhibit Ebp1 to stabilize p53, which in turn leads to G2 arrest and apoptosis in U87MG cells.


Vpr Proteome Apoptosis Cell cycle arrest p53 Ebp1 



This work was supported by Tianjin Science and Technology Committee (11JCYBJC12100, 12ZDZSY17700), the National Natural Science Foundation of China (81101911), Tianjin Health Bureau Science and Technique Foundation (11KG115), and State Key Clinical Department of Neurosurgery.

Conflict of interest

There are no conflicts of interest.


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Copyright information

© Federación de Sociedades Españolas de Oncología (FESEO) 2013

Authors and Affiliations

  1. 1.Tianjin Neurosurgical InstituteTianjin Huanhu HospitalTianjinPeople’s Republic of China
  2. 2.Clinical Lab of Tianjin Huanhu HospitalTianjinPeople’s Republic of China
  3. 3.Key Lab for Critical Care Medicine of the Ministry of HealthTianjin First Center HospitalTianjinPeople’s Republic of China

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