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Pantoea tagorei sp. nov., a Rhizospheric Bacteria with Plant Growth-Promoting Activities

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Abstract

A Gram-negative, short-rod, non-motile, facultatively anaerobic, potassium-solubilizing bacterium MR1 (Mine Rhizosphere) was isolated from rhizospheric soil of an open-cast coal mine of Jharia, Jharkhand, India. Isolate MR1 can grow in a broad range of temperature, pH, and NaCl concentrations. The 16S rRNA gene sequence of the strain showed 99.24% similarity with Pantoea septica LMG 5345T. However, maximum-likelihood tree constructed using 16S rRNA gene sequence, multilocus sequence analysis using concatenated sequences of ten housekeeping genes, whole-genome based phylogenetic reconstruction, digital DNA–DNA hybridization, and average nucleotide identity (ANIm and ANIb) values indicated segregation of MR1 from its closest relatives. Fatty acid profile of MR1 also suggested the same, with clear variation in major and minor fatty acid contents, having C13:0 anteiso (10-Methyldodecanoic acid) as the unique one. Thus, considering all polyphasic data, strain MR1T (= MTCC 13265T, where ‘T’ stands for Type strain) is presented as a novel species of the genus Pantoea, for which the name Pantoea tagorei sp. nov. is proposed.

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Data availability

The Whole Genome sequence has been deposited at DDBJ/ENA/GenBank under the Bioproject accession PRJNA892926.

Abbreviations

WGS:

Whole-genome sequencing

MLSA:

Multilocus sequence analysis

TYGS:

Type Strain Genome Server

dDDH:

Digital DNA–DNA hybridization

FAME:

Fatty acid methyl ester

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Acknowledgements

Raju Biswas, Abhinaba Chakraborty, and Puja Mukherjee, are grateful to WB-DST, SERB, and CSIR for their fellowships. Prof. Manoranjan Chowdhury (North Bengal University, India) is acknowledged for his suggestions in determining the taxonomic nomenclature of the novel species. Funding from DST-PURSE is acknowledged for FE-SEM imaging and analysis.

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  1. Raju Biswas and Arijit Misra have contributed equally.

Authors and Affiliations

  1. Microbiology Laboratory, Department of Botany (DST-FIST & UGC-DRS Funded), Institute of Science, Visva-Bharati (A Central University), Santiniketan, West Bengal, 731235, India

    Raju Biswas, Arijit Misra, Sandip Ghosh, Abhinaba Chakraborty, Puja Mukherjee & Bomba Dam

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  1. Raju Biswas
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BD, RB, and AM designed the study. RB, and AM analyzed the data. RB, AM, SG, AC, PM, and BD wrote the manuscript. All authors read and approved the final manuscript.

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Correspondence to Bomba Dam.

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Biswas, R., Misra, A., Ghosh, S. et al. Pantoea tagorei sp. nov., a Rhizospheric Bacteria with Plant Growth-Promoting Activities. Indian J Microbiol (2023). https://doi.org/10.1007/s12088-023-01147-9

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