Skip to main content

Advertisement

SpringerLink
Log in

Causatum of Probiotic Yeast Saccharomyces cerevisiae SBO1 Supplementation on Growth and Aflatoxin Amelioration in Broilers

  • ORIGINAL RESEARCH ARTICLE
  • Published:
Indian Journal of Microbiology Aims and scope Submit manuscript

Abstract

Probiotics are beneficial live microorganisms that benefit the host's health when administered in the required number. They play a vital role in preventing infectious diseases caused by pathogens. The current study aimed to discover a competent probiotic microbe that can detoxify aflatoxin and promote poultry health. The yeast isolate SBO1 tolerates the temperature of 42 °C, low pH, and high bile conditions, has good auto aggregation, hydrophobicity, and exhibits improved adherence to chick intestinal epithelial cells. In addition, it has an aflatoxin detoxifying ability of 56% after 24 h. In-vivo studies in broilers resulted in a higher body weight (2138 g) and greater feed conversion efficiency in the T2 group when fed with yeast SBO1-supplemented diet. Gizzard, spleen, and bursa Fabricius were all found to weigh the same, however, a significant difference (p < 0.05) was observed in the carcass, breast yield, and fat. Therefore it was determined that adding 0.2% yeast to the broiler diet increased performance by lessening the toxin's adverse effects.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3

Similar content being viewed by others

References

  1. Sun Z, Wang T, Demelash N, Zheng S, Zhao W, Chen X, Zhen Y, Qin G (2019) Effect of yeast culture (Saccharomyces cerevisiae) on broilers: a preliminary study on the effective components of yeast culture. Animals 10:68. https://doi.org/10.3390/ani10010068

    Article  PubMed  PubMed Central  Google Scholar 

  2. Velázquez-De Lucio BS, Hernández-Domínguez EM, Villa-Garcia M, Diaz-Godinez G, Mandujano-Gonzalez V, Mendoza-Mendoza B, Alvarez-Cervantes J (2021) Exogenous enzymes as zootechnical additives in animal feed: a review. Catalysts 11:851. https://doi.org/10.3390/catal11070851

    Article  CAS  Google Scholar 

  3. Bahaddad SA, Almalki MH, Alghamdi OA, Sohrab SS, Yasir M, Azhar EI, Chouayekh H (2022) Bacillus species as direct-fed microbial antibiotic alternatives for monogastric production. Probiotics Antimicrob Proteins. https://doi.org/10.1007/s12602-022-09909-5

    Article  PubMed  PubMed Central  Google Scholar 

  4. Jha R, Das R, Oak S, Mishra P (2020) Probiotics (direct-fed microbials) in poultry nutrition and their effects on nutrient utilization, growth and laying performance, and gut health: a systematic review. Animals 10:1863. https://doi.org/10.3390/ani10101863

    Article  PubMed  PubMed Central  Google Scholar 

  5. Bhukya KK, Bhukya B (2021) Unraveling the probiotic efficiency of bacterium Pediococcuspentosaceus OBK05 isolated from buttermilk: an in vitro study for cholesterol assimilation potential and antibiotic resistance status. PLoS ONE. https://doi.org/10.1371/journal.pone.0259702

    Article  PubMed  PubMed Central  Google Scholar 

  6. Kiarie EG, Leung H, Akbari Moghaddam Kakhki R, Patterson R, Barta JR (2019) Utility of feed enzymes and yeast derivatives in ameliorating deleterious effects of coccidiosis on intestinal health and function in broiler chickens. Front Vet Sci. https://doi.org/10.3389/fvets.00473

    Article  PubMed  PubMed Central  Google Scholar 

  7. Barigela A, Bhukya B (2021) Probiotic Pediococcusacidilactici strain from tomato pickle displays anti-cancer activity and alleviates gut inflammation in-vitro. 3 Biotech 1:1–11. https://doi.org/10.1007/s13205-020-02570-1

    Article  Google Scholar 

  8. Arif M, Iram A, Bhutta MA, Naiel MA, Abd El-Hack ME, Othman SI et al (2020) The biodegradation role of Saccharomyces cerevisiae against harmful effects of mycotoxin contaminated diets on broiler performance, immunity status, and carcass characteristics. Animals 10:238

    Article  PubMed  PubMed Central  Google Scholar 

  9. Kumar A, Pathak H, Bhadauria S, Sudan J (2021) Aflatoxin contamination in food crops: causes, detection, and management—a review. Food Prod Process Nutr 3:1–9. https://doi.org/10.1186/s43014-021-00064-y

    Article  CAS  Google Scholar 

  10. Guan Y, Chen J, Nepovimova E, Long M, Wu W, Kuca K (2021) Aflatoxin detoxification using microorganisms and enzymes. Toxins 13:46. https://doi.org/10.3390/toxins.13010046

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  11. Colovic R, Puvača N, Cheli F, Avantaggiato G, Greco D, Đuragić O, Kos J, Pinotti L (2019) Decontamination of mycotoxin-contaminated feedstuffs and compound feed. Toxins 11:617. https://doi.org/10.3390/toxins11110617

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  12. Piotrowska M (2021) Microbiological decontamination of mycotoxins: opportunities and limitations. Toxins 13:819. https://doi.org/10.3390/toxins13110819

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  13. Qvirist LA, De Filippo C, Strati F, Stefanini I, Sordo M, Andlid T et al (2016) Isolation, identification and characterization of yeasts from fermented goat milk of the Yaghnob Valley in Tajikistan. Front Microbiol 7:1690. https://doi.org/10.3389/fmicb.2016.01690

    Article  PubMed  PubMed Central  Google Scholar 

  14. Somashekaraiah R, Shruthi B, Deepthi BV, Sreenivasa MY (2019) Probiotic properties of lactic acid bacteria isolated from neera: a naturally fermenting coconut palm nectar. Front Microbiol. https://doi.org/10.3389/fmicb.2019.01382

    Article  PubMed  PubMed Central  Google Scholar 

  15. Deepak MB, Jhanvi SP, Anu Appaiah KA (2015) Aflatoxin binding and detoxification by non-saccharomyces yeast a new vista for decontamination. Int J Curr Microbiol App Sci 4:310–317

    CAS  Google Scholar 

  16. Zolfaghari H, Khezerlou A, Ehsani A, Khosroushahi AY (2020) Detoxification of aflatoxin B1 by probiotic Yeasts and Bacteria isolated from dairy products of Iran. Adv Pharma Bull 10:482. https://doi.org/10.34172/apb.2020.060

    Article  CAS  Google Scholar 

  17. Haldar S, Ghosh TK, Bedford MR (2011) Effects of yeast (Saccharomyces cerevisiae) and yeast protein concentrate on production performance of broiler chickens exposed to heat stress and challenged with Salmonella enteritidis. Anim Feed Sci Technol 168:61–71. https://doi.org/10.1016/j.anifeedsci.03.007

    Article  CAS  Google Scholar 

  18. Rao KR, Vipin AV, Hariprasad P, Appaiah KA, Venkateswaran GJFC (2017) Biological detoxification of aflatoxin B1 by Bacillus licheniformis CFR1. Food Control 71:234–241. https://doi.org/10.1016/j.foodcont.06.040

    Article  Google Scholar 

  19. Kumar S, Stecher G, Li M, Knyaz C, Tamura K (2018) MEGA X: molecular evolutionary genetics analysis across computing platforms. Mol Biol Evol 35:1547–1549. https://doi.org/10.1093/molbev/msy096

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  20. Bortoluzzi C, Barbosa JGM, Pereira R, Fagundes NS, Rafael JM, Menten JFM (2018) Autolyzed yeast (Saccharomyces cerevisiae) supplementation improves performance while modulating the intestinal immune-system and microbiology of broiler chickens. Front Sustain Food Syst. https://doi.org/10.3389/fsufs.2018.00085

    Article  Google Scholar 

  21. Srinivas B, Rani GS, Kumar BK, Chandrasekhar B, Krishna KV, Devi TA, Bhima B (2017) Evaluating the probiotic and therapeutic potentials of Saccharomyces cerevisiae strain (OBS2) isolated from fermented nectar of toddy palm. AMB Express 7:1–14. https://doi.org/10.1186/s13568-016-0301-1

    Article  CAS  Google Scholar 

  22. Pizzolitto RP, Armando MR, Combina M, Cavaglieri LR, Dalcero AM, Salvano MA (2012) Evaluation of Saccharomyces cerevisiae strains as probiotic agent with aflatoxin B1 adsorption ability for use in poultry feedstuffs. J Environ Sci Health B 47:933–941. https://doi.org/10.1080/03601234.2012.706558

    Article  CAS  PubMed  Google Scholar 

  23. Hossain MN, Afrin S, Humayun S, Ahmed MM, Saha BK (2020) Identification and growth characterization of a novel strain of Saccharomyces boulardii isolated from soya paste. Front Nutr 7:27. https://doi.org/10.3389/fnut.2020.00027

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  24. Romero-Luna HE, Hernández-Sánchez H, Ribas-Aparicio RM, Cauich-Sánchez PI, Dávila-Ortiz G (2019) Evaluation of the probiotic potential of Saccharomyces cerevisiae Strain (C41) isolated from Tibicos by in vitro studies. Probiotics Antimicrob Proteins 11:794–800. https://doi.org/10.1007/s12602-018-9471-2

    Article  CAS  PubMed  Google Scholar 

  25. Gotcheva V, Hristozova E, Hristozova T, Guo M, Roshkova Z, Angelov A (2002) Assessment of potential probiotic properties of lactic acid bacteria and yeast strains. Food Biotechnol 16:211–225. https://doi.org/10.1081/FBT.120016668

    Article  Google Scholar 

  26. Chuang WY, Lin WC, Hsieh YC, Huang CM, Chang SC, Lee TT (2019) Evaluation of the combined use of Saccharomyces cerevisiae and Aspergillus oryzae with phytase fermentation products on growth, inflammatory, and intestinal morphology in broilers. Animals 9:1051. https://doi.org/10.3390/ani9121051

    Article  PubMed  PubMed Central  Google Scholar 

  27. Shankar PA, Premavalli K, Omprakash AV, Kirubakaran JJ, Hudson GH (2017) Effect of dietary yeast supplementation on the production performance of broilers. Int J Adv Biol Res 7:222–228

    CAS  Google Scholar 

  28. Fathi MM, Al-Mansour S, Al-Homidan A, Al-Khalaf A, Al-Damegh M (2012) Effect of yeast culture supplementation on carcass yield and humoral immune response of broiler chicks. Vet World 5:651. https://doi.org/10.5455/vetworld.2012.651-657

    Article  Google Scholar 

  29. Adebiyi OA, Makanjuola BA, Bankole TO, Adeyori AS (2012) Yeast culture (Saccharomyces cerevisae) supplementation: effect on the performance and gut morphology of broiler birds. Glob J Sci Front Res Biol Sci 12:25–29

    Google Scholar 

  30. Pizzolitto RP, Armando MR, Salvano MA, Dalcero AM, Rosa CA (2013) Evaluation of Saccharomyces cerevisiae as an antiaflatoxicogenic agent in broiler feedstuffs. Poult Sci 92:1655–1663. https://doi.org/10.3382/ps.2012-02846

    Article  CAS  PubMed  Google Scholar 

  31. Stanley VG, Ojo R, Woldesenbet S, Hutchinson DH, Kubena LF (1993) The use of Saccharomyces cerevisiae to suppress the effects of aflatoxicosis in broiler chicks. Poult Sci 72:1867–1872. https://doi.org/10.3382/ps.0721867

    Article  CAS  PubMed  Google Scholar 

  32. Gao J, Zhang HJ, Yu SH, Wu SG, Yoon I, Quigley J (2008) Effects of yeast culture in broiler diets on performance and immunomodulatory functions. Poult Sci 87:1377–1384. https://doi.org/10.3382/ps.2007-00418

    Article  CAS  PubMed  Google Scholar 

  33. Onifade AA, Odunsi AA, Babatunde GM, Olorede BR, Muma E (1999) Comparison of the supplemental effects of Saccharomyces cerevisiae and antibiotics in low-protein and high-fibre diets fed to broiler chickens. Arch Anim Nutr 52:29–39. https://doi.org/10.1080/17450399909386149

    Article  CAS  Google Scholar 

  34. Kumar S, Yadav SP, Chandra G, Sahu DS, Kumar R, Maurya PS (2019) Effect of dietary supplementation of yeast (Saccharomyces cerevisiae) on performance and hemato-biochemical status of broilers. Indian J Poult Sci 54:15–19. https://doi.org/10.5958/0974-8180.00002.3

    Article  Google Scholar 

  35. Zhen YG, Zhao W, Zhang XF, Li LJ, Chen X, Wang T, Lee HG (2019) Effects of yeast culture on broiler growth performance, nutrient digestibility and caecal microbiota. S Afr J Anim Sci 49:99–108. https://doi.org/10.4314/sajas.v49i1.12

    Article  CAS  Google Scholar 

  36. Corduk M, Ceylan N, Dede N, Tel OY (2008) Effects of novel feed additives on performance, carcass traits and E. coli, aerobic bacteria and yeast counts in broilers. Arch Geflugelkd 72:61–67

    CAS  Google Scholar 

  37. Yalçın S, Eser H, Cengiz S, Eltan Ö (2013) Effects of dietary yeast autolysate (Saccharomyces cerevisiae) on performance, carcass and gut characteristics, blood profile, and antibody production to sheep red blood cells in broilers. J Appl Poult Res 22:55–61. https://doi.org/10.3382/japr.2012-00577

    Article  CAS  Google Scholar 

Download references

Acknowledgements

Authors are grateful to the Department of Poultry Science, P.V. Narsimha Rao Telangana State Veterinary University (PVNRTVU) for providing facility and assistance during poultry studies.

Funding

Financial support was obtained from the Ministry of Human Resource Development, Govt. India under RUSA 2.0 program, Science and Engineering Research board (SB/EMEQ-258/2013) and University Grants Commission, BSR-RFSMS, Govt. of India to carry out the research work.

Author information

Authors and Affiliations

  1. Centre for Microbial and Fermentation Technology, Department of Microbiology, University College of Science, Osmania University, Hyderabad, Telangana State, 500007, India

    Swaruparani Ganapathiwar & Bhima Bhukya

  2. Department of Poultry Science, College of Veterinary Science, P. V. Narsimha Rao Telangana Veterinary University, Rajendranagar, Hyderabad, Telangana State, 500030, India

    Radhika Pappula

  3. Department of Veterinary Pharmacology and Toxicology, College of Veterinary Science, P. V. Narsimha Rao Telangana Veterinary University, Rajendranagar, Hyderabad, Telangana State, 500030, India

    Anil Kumar Banothu

Authors
  1. Swaruparani Ganapathiwar
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Radhika Pappula
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Anil Kumar Banothu
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Bhima Bhukya
    View author publications

    You can also search for this author in PubMed Google Scholar

Contributions

Manuscript preparation and all the experiments of the manuscript were carried out by SG. RP helped in feed preparation, maintenance of birds and manuscript writing. AKB monitored and supervised the poultry experiments. BB, designed the concept, and monitored the experiments and the corresponding author. All the authors approved the manuscript.

Corresponding author

Correspondence to Bhima Bhukya.

Additional information

Publisher's Note

Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.

Supplementary Information

Below is the link to the electronic supplementary material.

Supplementary file1 (DOCX 67 kb)

Rights and permissions

Springer Nature or its licensor (e.g. a society or other partner) holds exclusive rights to this article under a publishing agreement with the author(s) or other rightsholder(s); author self-archiving of the accepted manuscript version of this article is solely governed by the terms of such publishing agreement and applicable law.

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Ganapathiwar, S., Pappula, R., Banothu, A.K. et al. Causatum of Probiotic Yeast Saccharomyces cerevisiae SBO1 Supplementation on Growth and Aflatoxin Amelioration in Broilers. Indian J Microbiol 63, 253–262 (2023). https://doi.org/10.1007/s12088-023-01078-5

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s12088-023-01078-5

Keywords

Search

Navigation