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Prevalence, Antimicrobial Susceptibility and Resistance Gene Detection in Bacteria Isolated from Goldfish and Tiger Barb from Ornamental Fish Farms of Tamil Nadu

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Abstract

This study aims to determine the antimicrobial resistance (AMR) pattern in freshwater ornamental cyprinids, such as Goldfish and Tiger barb. Molecular characterization of bacterial isolates confirmed the presence of 7 bacterial isolates in Goldfish and 6 in Tiger barb. Antimicrobial susceptibility test using 36 antibiotics revealed a higher resistance pattern for bacitracin, rifampicin, trimethoprim, cefalexin, ampicillin, amoxicillin, nalidixic acid and nitrofurantoin. Sulphafurazole, norfloxacin and ciprofloxacin were effective against all the bacterial isolates derived from Goldfish and Tiger barb. Most bacterial isolates exhibited > 0.2 multi-drug resistance index (MDR), indicating the severity of antibiotic use in the culture system. The finding of the present study suggests that ornamental fish may act as the reservoir of MDR bacteria and dissemination of resistance genes to clinical and human commensal bacteria through horizontal gene transfer.

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Acknowledgements

The authors acknowledge the Institute of Fisheries Post Graduate Studies, TNJFU for the support.

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There is no specific grant for this publication.

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Author notes

  1. Ayyathurai Kathirvelpandian

    Present address: PMFGR Centre, ICAR-National Bureau of Fish Genetic Resources, Kochi, Kerala, 682018, India

Authors and Affiliations

  1. Institute of Fisheries Post Graduate Studies, Tamil Nadu Dr. J. Jayalalithaa Fisheries University, Vaniyanchavadi, Chennai, Tamil Nadu, 603103, India

    Nallaiah Hemamalini, Seerappalli Aran Shanmugam, Ayyathurai Kathirvelpandian, Agarwal Deepak, Venkatachalam Kaliyamurthi & Eswaran Suresh

  2. Dr. M.G.R. Fisheries College and Research Institute, Ponneri, Tamil Nadu, 601204, India

    Selvaram Ezhilmathi

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  1. Nallaiah Hemamalini
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Contributions

N.H. performed the work as part of her Ph. D. research work and drafted the manuscript; S.A.S. provided technical support, and provided overall guidance; A.D. analyzed the data; V.K assisted in revising the manuscript; E.S. and S.E. extended help with data acquisition and revising the manuscript critically for important intellectual content; A.K. conceptualized and designed the experiment.

Corresponding author

Correspondence to Nallaiah Hemamalini.

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The authors declare no conflict of interest.

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Tamil Nadu Dr. J. Jayalalithaa Fisheries University, Nagapattinam, has approved the procedures and methodologies followed in the present study.

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Hemamalini, N., Shanmugam, S.A., Kathirvelpandian, A. et al. Prevalence, Antimicrobial Susceptibility and Resistance Gene Detection in Bacteria Isolated from Goldfish and Tiger Barb from Ornamental Fish Farms of Tamil Nadu. Indian J Microbiol 62, 441–446 (2022). https://doi.org/10.1007/s12088-022-01023-y

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  • DOI: https://doi.org/10.1007/s12088-022-01023-y

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