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HPLC Method Validated for Quantification of Fluconazole Co-Encapsulated with Propolis Within Chitosan Nanoparticles

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Abstract

Considering the cases of fungal resistance to classic antifungals, it is necessary to develop more efficient and innovative therapies capable of reversing this situation. Fluconazole is an antifungal frequently used in the treatment of mycosis and some fungi developed resistance to its mechanism of action. In this work, fluconazole and green propolis were co-encapsulated in chitosan nanoparticles to be explored in order to promote a synergistic effect to enhance its therapeutic efficacy. However, because of the complexity of the chemical composition of green propolis, it was necessary to develop a simple and precise methodology to quantify fluconazole in the formulation. High Efficiency Liquid Chromatography methodology was developed and validated following the Brazilian regulatory guidelines (ANVISA, RDC 166/2017) for the separation of co-eluted peaks of fluconazole and green propolis in the nanoparticle supernatant. Applying the method developed, it was possible to quantify fluconazole in the same sample containing propolis. Thus, the results allow to affirm that it is a specific test, effective, precise and robust, which helped to determine the efficiency of association of the compounds within the nanoparticle. The method can be applied to quantify compounds that have similar chromatographic retention times.

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Acknowledgements

The authors thank the Coordination for the Improvement of Higher Education Personnel (CAPES) for Silva JT scholarship and Federal University of Goiás for providing infrastructural facilities.

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Authors and Affiliations

  1. Laboratory of Nano & Biotechnology, Instituto de Patologia Tropical E Saúde Pública, Universidade Federal de Goiás, Rua 235, s/ n, Setor Universitário, Goiânia, GO, Brazil

    Jacqueline Teixeira da Silva & Andre Correa Amaral

  2. Faculty of Pharmacy, Universidade Federal de Goiás, Goiânia, GO, Brazil

    Matheus Gabriel de Oliveira & José Realino de Paula

  3. School of Medical Sciences, Pharmaceuticals and Biomedical Sciences, Pontifícia Universidade Católica de Goiás, Goiânia, GO, Brazil

    Suzana Ferreira Alves

  4. Company Citrinitas, São Paulo, SP, Brazil

    Flavio Pellegrini

Authors
  1. Jacqueline Teixeira da Silva
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  2. Matheus Gabriel de Oliveira
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  3. José Realino de Paula
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  4. Suzana Ferreira Alves
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  5. Flavio Pellegrini
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  6. Andre Correa Amaral
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Correspondence to Andre Correa Amaral.

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da Silva, J.T., de Oliveira, M.G., de Paula, J.R. et al. HPLC Method Validated for Quantification of Fluconazole Co-Encapsulated with Propolis Within Chitosan Nanoparticles. Indian J Microbiol 61, 364–369 (2021). https://doi.org/10.1007/s12088-021-00954-2

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  • DOI: https://doi.org/10.1007/s12088-021-00954-2

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