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Heavy Metals Scavenging Potential of Trichoderma asperellum and Hypocrea nigricans Isolated from Acid Soil of Jharkhand

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Abstract

Trichoderma asperellum (NAIMCC-F-03167) and Hypocrea nigricans (NAIMCC-F-03168) were isolated from the acidic soil of the vicinity of Litchi orchard, Ranchi, Jharkhand and were characterized on the basis of morphological, molecular and biochemical features. Both strains are fast growing, light to dark green, highly sporulative and have ability to cover 90 mm Petri dish within 96 h of inoculation. Biochemcial estimation of both isolates indicated significant cellulase and phosphate solubilisation activity. Highest cellulase activity was observed in T. asperellum (5.63 cm) followed by H. nigricans (5.10 cm) and phosphate solubilisation index was observed maximum in T. asperellum (1.93) followed by H. nigricans (1.39). Moreover, these isolates were molecularly identified on the basis of ribosomal DNA based sequences database and phylogenetic analysis in NCBI GenBank as T. asperellum (NCBI-KM 438015) and H. nigricans (NCBI-KJ910335). Negetive effect on sporulation of Lead (Pb) and Cadmium (Cd) was observed while in heavy metal scavenging potential, T. asperellum (88.9% Cd) showed highest scavenging potential followed by H. nigricans (87.2% Cd) while in Pb scavenging potential, H. nigricans (88% Pb) followed highest scavenging potential followed by T. asperellum (81.30% Pb) after 21 days of inoculation from 30 µg/ml heavy metals concentrated broth medium. If both potential bioagents can apply in Cd and Pb affected soil/water will be helpful in scavenging of heavy metals as well as management of phosphorus deficiency and soilborne fungal diseases.

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References

  1. Kristanti RA, Hadibarata T, Toyama T, Tanaka Y, Kazuhiro M (2011) Bioremediation of crude oil by white rot fungi Polyporus sp. S133. J Microbiol Biotechnol 21:995–1000. https://doi.org/10.4014/jmb.1105.05047

    Article  CAS  PubMed  Google Scholar 

  2. Sarma H (2011) Metal hyper accumulation in plants: a review focusing on phytoremediation technology. J Environ Sci Technol 4:118–138. https://doi.org/10.3923/jest.2011.118.138

    Article  CAS  Google Scholar 

  3. Singh R, Gautam N, Mishra A, Gupta R (2011) Heavy metals and living system: an overview. Indian J Pharmacol 43:246–253. https://doi.org/10.4103/0253-7613.81505

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  4. Alvarez-Ayuso E (2008) Cadmium in soil-plant systems: an overview. Int J Environ Pollut 33:275–291. https://doi.org/10.1504/IJEP.2008.019399

    Article  CAS  Google Scholar 

  5. Jain S, Bhatt A (2014) Molecular and In situ characterization of cadmium-resistant diversified extremophilic strains of Pseudomonas for their bioremediation potential. Biotech 4:297–304. https://doi.org/10.1007/s13205-013-0155-z

    Article  Google Scholar 

  6. Sethy SK, Ghosh S (2013) Effect of heavy metals on germination of seeds. J Nat Sci Biol Med 4:272–275. https://doi.org/10.4103/0976-9668.116964

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  7. Zaltauskaite J, Sodiene I (2010) Effects of total cadmium and lead concentrations in soil on the growth, reproduction and survival of earthworm Eisenia fetida. Ekologija 56:10–16. https://doi.org/10.2478/v10055-010-0002-z

    Article  CAS  Google Scholar 

  8. Ogundele DT, Adio AA, Oludele OE (2015) Heavy metal concentrations in plants and soil along heavy traffic roads in North Central Nigeria. J Environ Anal Toxicol 5:334. https://doi.org/10.4172/2161-0525.1000334

    Article  Google Scholar 

  9. Krishnaveni M, Kumar JS, Shravanan PS (2015) Influence of Lead on biochemicals and proline contents of Vigna unguiculata (L.) Walp. Int J Plant Sci 10:142–151. https://doi.org/10.15740/HAS/IJPS/10.2/142-151

    Article  Google Scholar 

  10. Srinivasan M, Sahi SV, Paulo JCF, Venkatachalam P (2016) Lead heavy metal toxicity induced changes on growth and antioxidative enzymes level in water hyacinths [Eichhornia crassipes (Mart.)]. Bot Stud 55:54. https://doi.org/10.1186/s40529-014-0054-6

    Article  CAS  Google Scholar 

  11. Sharma P, Dubey RS (2005) Lead toxicity in plants. Braz J Plant Physiol 17:35–52. https://doi.org/10.1590/S1677-04202005000100004

    Article  CAS  Google Scholar 

  12. Verma S, Dubey RS (2003) Lead toxicity induces lipid peroxidation and alters the activities of antioxidants enzymes in growing rice plants. Plant Sci 164:645–655. https://doi.org/10.1016/S0168-9452(03)00022-0

    Article  CAS  Google Scholar 

  13. Aliu S, Gashi B, Rusinovci I, Fetahu S, Vataj R (2013) Effects of some heavy metals in some morpho-physiological parameters in maize seedlings. Am J Biochem Biotech 9:27–33. https://doi.org/10.3844/ajbbsp.2013.27.33

    Article  CAS  Google Scholar 

  14. Ling T, Fangke Y, Jun R (2010) Effect of Mercury to seed germination, coleoptile growth and root elongation of four vegetables. Res J Phytochem 4:225–233. https://doi.org/10.3923/rjphyto.2010.225.233

    Article  CAS  Google Scholar 

  15. Rajasulochana P, Preethy V (2016) Comparison on efficiency of various techniques in treatment of waste and sewage water—a comprehensive review. Resour Eff Technol 2:175–184. https://doi.org/10.1016/j.reffit.2016.09.004

    Article  Google Scholar 

  16. Mishra A, Malik A (2004) Recent advances in microbial metal bioaccumulation. J Crit Rev Env Sci Tech 43:1162–1222. https://doi.org/10.1080/10934529.2011.627044

    Article  CAS  Google Scholar 

  17. Gupta A, Joia J, Sood A, Sood R, Sidhu C, Kaur G (2016) Microbes as potential tool for remediation of heavy metals: a review. J Microb Biochem Technol 8:364–372. https://doi.org/10.4172/1948-5948.1000310

    Article  CAS  Google Scholar 

  18. Pilon-Smits E (2005) Phytoremediation. Annu Rev Plant Biol 56:15–39. https://doi.org/10.1146/annurev.arplant.56.032604.144214

    Article  CAS  PubMed  Google Scholar 

  19. Aken BV, Correa PA, Schnoor JL (2010) Phytoremediation of polychlorinated biphenyls: new trends and promises. Environ Sci Technol 44:2767–2776. https://doi.org/10.1021/es902514d

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  20. Petrovic JJ, Danilovic G, Curcic N, Milinkovic M, Stosic N, Pankovic D, Raicevic Vera (2014) Copper tolerance of Trichoderma species. Arch Biol Sci Belgrade 66:137–142. https://doi.org/10.2298/ABS1401137J

    Article  Google Scholar 

  21. Cramer RA, Byrne PF, Brick MA, Panella L, Wickliffe E, Schwartz HF (2003) Characterization of Fusarium oxysporum isolates from common bean and sugar beet using pathogenicity assays and random-amplified polymorphic DNA markers. J Phytopathol 151:352–360. https://doi.org/10.1046/j.1439-0434.2003.00731.x/pdf

    Article  Google Scholar 

  22. Borneman J, Hartin RJ (2000) PCR primers that amplify fungal rRNA genes from environmental samples. Appl Environ Microbiol 66:4356–4360. https://doi.org/10.1128/AEM.66.10.4356-4360.2000

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  23. Saitou N, Nei M (1987) The neighbour-joining method: a new method for reconstructing phylogenetic trees. Mol Biology Evol 4:406–425. https://doi.org/10.1093/oxfordjournals.molbev.a040454

    Article  CAS  Google Scholar 

  24. Felsenstein J (1985) Confidence limits on phylogenies: an approach using the bootstrap. Evolution 39:783–791. https://doi.org/10.1111/j.1558-5646.1985.tb00420.x

    Article  PubMed  Google Scholar 

  25. Tamura K, Stecher G, Peterson D, Filipski A, Kumar S (2013) MEGA6: molecular evolutionary genetics analysis version 6.0. Mol Biol Evol 30:2725–2729. https://doi.org/10.1093/molbev/mst197

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  26. Vincent JM (1947) Distortion of fungal hyphae in presence of certain inhibitors. Nature 159:850. https://doi.org/10.1038/159850b0

    Article  CAS  PubMed  Google Scholar 

  27. Johnsen HR, Krause K (2014) Cellulase activity screening using pure carboxymethyl cellulose: application to soluble cellulolytic samples and to plant tissue prints. Int J Mol Sci 15:830–838. https://doi.org/10.3390/ijms15010830

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  28. Gadd GM (2009) Biosorption: critical review of scientific rationale, environmental importance and significance for pollution treatment. J Chem Technol Biotechnol 84:13–28. https://doi.org/10.1002/jctb.1999

    Article  CAS  Google Scholar 

  29. Harman GE, Howell CR, Viterbo A, Chet I, Lorito M (2004) Trichoderma species—opportunistic, avirulent plant symbionts. Nat Rev Microbiol 2:43–56. https://doi.org/10.1038/nrmicro797

    Article  CAS  PubMed  Google Scholar 

  30. Harman GE (2006) Overview of mechanisms and uses of Trichoderma sp. Phytopathol 96:190–194. https://doi.org/10.1094/PHYTO-96-0190

    Article  CAS  Google Scholar 

  31. Schuster A, Schmoll M (2010) Biology and biotechnology of Trichoderma. Appl Microbiol Biotechnol 87:787–799. https://doi.org/10.1007/s00253-010-2632-1

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  32. Khaledi N, Taheri P (2016) Biocontrol mechanisms of Trichoderma harzianum against soybean charcoal rot caused by Macrophomina phaseolina. J Plant Prot Res 56:21–31. https://doi.org/10.1515/jppr-2016-0004

    Article  CAS  Google Scholar 

  33. Guilger M, Pasquoto-Stigliani T, Bilesky-Jose N, Grillo R, Abhilash PC, Fernandes FL, de Lima R (2017) Biogenic silver nanoparticles based on Trichoderma harzianum: synthesis, characterization, toxicity evaluation and biological activity. Sci Rep 7:44421. https://doi.org/10.1038/srep44421

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  34. Rahman A, Begum D, Rahman M, Bari MA, Ilias GNM, Alam MF (2011) Isolation and identification of Trichoderma species and their use of bioconversion of solid waste. Turk J Biol 35:183–194. https://doi.org/10.3906/biy-0905-8

    Article  Google Scholar 

  35. Raja HA, Miller AN, Pearce CJ, Oberlies NH (2017) Fungal identification using molecular tools: a primer for the natural products research community. J Nat Prod 80:756–770. https://doi.org/10.1021/acs.jnatprod.6b01085

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  36. Bruns TD, White TJ, Taylor JW (1991) Fungal molecular systematic. Annu Rev Ecol Syst 22:525–564. https://doi.org/10.1146/annurev.es.22.110191.002521

    Article  Google Scholar 

  37. Freeman J, Ward E (2004) Gaeumannomyces graminis, the take-all fungus and its relatives. Mol Plant Pathol 5:235–252. https://doi.org/10.1111/j.1364-3703.2004.00226.x

    Article  CAS  PubMed  Google Scholar 

  38. Kimura M (1980) A simple method for estimating evolutionary rate of base substitutions through comparative studies of nucleotide sequences. J Mol Evol 16:111–120. https://doi.org/10.1007/BF01731581

    Article  CAS  PubMed  Google Scholar 

  39. Paranthaman SR, Karthikeyen B (2015) Bioremediation of heavy metal in paper mill effluents using Pseudomonas spp. Int J Microbiol 1:1–5

    Google Scholar 

  40. Chaturvedi AD, Pal D, Penta S, Kumar A (2015) Ecotoxic heavy metals transformation by bacteria and fungi in aquatic ecosystem. World J Microbiol Biotechnol 31:1595–1603. https://doi.org/10.1007/s11274-015-1911-5

    Article  CAS  PubMed  Google Scholar 

  41. Ayangbenro AS, Babalola OO (2017) A new strategy for heavy metal polluted environments: a review of microbial biosorbents. Int J Environ Res Public Health 14:94. https://doi.org/10.3390/ijerph14010094

    Article  CAS  PubMed Central  Google Scholar 

  42. Naz N, Young HK, Ahmed N, Gadd GM (2005) Cadmium accumulation and DNA homology with metal resistance genes in sulfate-reducing bacteria. Appl Environ Microbiol 71:4610–4618. https://doi.org/10.1128/AEM.71.8.4610-4618.2005

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  43. Cai C-X, Xu J, Deng N-F, Dong X-W, Tang H, Liang Y, Fan X-W, Li Y-Z (2016) A novel approach of utilization of the fungal conidia biomass to remove heavy metals from the aqueous solution through immobilization. Sci Rep 6:36546. https://doi.org/10.1038/srep36546

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  44. Anand P, Isar J, Saran S, Saxena RK (2006) Bioaccumulation of copper by Trichoderma viride. Bioresour Technol 97:1018–1025. https://doi.org/10.1016/j.biortech.2005.04.046

    Article  CAS  PubMed  Google Scholar 

  45. Ng IS, Tsai SW, Ju YM, Yu SM, Ho TH (2011) Dynamic synergistic effect on Trichoderma reesei cellulases by novel beta-glucosidases from Taiwanese fungi. Bioresour Technol 102:6073–6081. https://doi.org/10.1016/j.biortech.2010.12.110

    Article  CAS  PubMed  Google Scholar 

  46. Ng IS, Wu X, Yang X, Xie Y, Lu Y, Chen C (2013) Synergistic effect of Trichoderma reesei cellulases on agricultural tea waste for adsorption of heavy metals Cr(VI). Bioresour Technol 145:297–301. https://doi.org/10.1016/j.biortech.2013.01.105

    Article  CAS  PubMed  Google Scholar 

  47. Malaviya P, Singh A (2014) Bioremediation of chromium solutions and chromium containing wastewaters. J Critical Rev Microbiol 42:607–633. https://doi.org/10.3109/1040841X.2014.974501

    Article  CAS  Google Scholar 

  48. Li H, Li Z, Liu T, Xiao X, Peng Z, Deng L (2008) A novel technology for biosorption and recovery hexavalent chromium in wastewater by biofunctional magnetic beads. Bioresour Technol 99:6271–6279

    Article  CAS  PubMed  Google Scholar 

  49. Mohan D, Rajput S, Singh VK, Steele PH, Pittman CU Jr (2010) Modelling and evaluation of chromium remediation from water using low cost bio-char, a green adsorbent. J Hazard Mater 188:319–333. https://doi.org/10.1016/j.jhazmat.2011.01.127

    Article  CAS  Google Scholar 

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Acknowledgements

The authors are grateful to Dr. B. P. Bhatt, Director, ICAR-Research Complex for Eastern Region, Patna, India, for providing facility for doing this research is duly acknowledged. Authors are also thankful to Dr. S. B. Choudhary, NBPGR, Ranchi, Jharkhand for critical review of the manuscript.

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Funding was provided by Indian Council of Agricultural Research, New Delhi, India.

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Correspondence to Sudarshan Maurya.

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Maurya, S., Rashk-E-Eram, Naik, S.K. et al. Heavy Metals Scavenging Potential of Trichoderma asperellum and Hypocrea nigricans Isolated from Acid Soil of Jharkhand. Indian J Microbiol 59, 27–38 (2019). https://doi.org/10.1007/s12088-018-0756-7

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