Indian Journal of Microbiology

, Volume 58, Issue 3, pp 312–318 | Cite as

Oral Intake of Lactobacillus helveticus NS8 Alleviates Ovalbumin-Induced Atopic Dermatitis in SKH-1 Hairless Mice

  • Jingjing Rong
  • Shuzhan Liu
  • Chao Hu
  • Feng Jin
  • Li Wang
Original Research Article


Atopic dermatitis (AD) is the most common chronic inflammatory skin disease and is driven by strong type 2 immune responses. Lactobacillus helveticus NS8 (NS8), a probiotic strain isolated from Mongolian koumiss, has anti-inflammatory activities. Here, we evaluated the therapeutic potential of NS8 on AD-like skin lesions by using SKH-1 hairless mice that underwent three cycles of epicutaneous sensitization (EC) with ovalbumin (OVA). NS8 (5 × 108 CFU/day) was orally administered to mice from 2 weeks before the first sensitization until the end of the study. NS8 attenuated the symptoms and pathological changes in the skin of AD mice. For example, NS8 reduced epidermal and dermal thickening and significantly restrained the infiltration of mast cells, eosinophils, and CD4+ T cells into the dermis. By analysing the Th1/Th2 cytokines produced in skin lesions, we found that NS8 significantly suppressed the expression of IL-4, IL-5, and IL-13 (P < 0.05), while it had no discernible effect on the expression of IFN-γ. Systemically, NS-8 reduced the total IgE and OVA-specific IgE levels in serum (P < 0.05). Our study demonstrates that oral administration of L. helveticus NS8 effectively alleviates AD severity in mice by suppressing the Th2 immune response. NS8 may be a promising candidate for prophylactic and therapeutic treatments of allergic diseases, such as AD.


Atopic dermatitis Probiotic Lactobacillus helveticus Th1/Th2 balance IgE 



This research has been supported by Natural Science Foundation of China (Nos. 81472659 and 81101560).

Compliance with Ethical Standards

Conflict of interest

The authors declare that they have no competing interests.


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Copyright information

© Association of Microbiologists of India 2018

Authors and Affiliations

  • Jingjing Rong
    • 1
    • 2
  • Shuzhan Liu
    • 2
  • Chao Hu
    • 2
  • Feng Jin
    • 3
  • Li Wang
    • 2
  1. 1.Key Laboratory of Zoological Systematics and Evolution, Institute of ZoologyChinese Academy of SciencesBeijingChina
  2. 2.Institute of Ageing Research, School of MedicineHangzhou Normal UniversityHangzhouChina
  3. 3.Key Lab of Mental Health, Institute of PsychologyChinese Academy of SciencesBeijingChina

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