Indian Journal of Microbiology

, Volume 58, Issue 3, pp 301–311 | Cite as

Comparison of Antimicrobial Properties of Silver Nanoparticles Synthesized from Selected Bacteria

  • M. M. K. Peiris
  • S. S. N. Fernando
  • P. M. Jayaweera
  • N. D. H. Arachchi
  • T. D. C. P. Guansekara
Original Research Article


Green silver nanoparticle (AgNP) biosynthesis is facilitated by the enzyme mediated reduction of Ag ions by plants, fungi and bacteria. The antimicrobial activity of green AgNPs is useful to overcome the challenge of antimicrobial resistance. Antimicrobial properties of biosynthesized AgNPs depend on multiple factors including culture conditions and the microbial source. The antimicrobial activity of AgNPs biosynthesized by Pseudomonas aeruginosa ATCC 27853, Escherichia coli ATCC 25922, Staphylococcus aureus ATCC 25923 and Acinetobacter baumannii (confirmed clinical isolate) were investigated in this study. Biosynthesis conditions (AgNO3 concentration, pH, incubation temperature and incubation time) were optimized to obtain the maximum AgNP yield. Presence of AgNPs was confirmed by observing a characteristic UV–Visible absorbance peak in 420–435 nm range. AgNP biosynthesis was optimal at 0.4 g/L AgNO3 concentration under alkaline conditions at 60–70 °C. The biosynthesized AgNPs showed higher stability compared to chemogenized AgNPs in the presence of electrolytes. AgNPs synthesized by P. aeruginosa were the most stable while NPs of S. aureus were the least stable. AgNPs synthesized by P. aeruginosa and S. aureus showed good antimicrobial potential against E. coli, P. aeruginosa, S. aureus, MRSA and Candida albicans. AgNPs synthesized by S. aureus had greater antimicrobial activity. The antimicrobial activity of NPs may vary depending on the size and the morphology of NPs.


Silver nanoparticle Antimicrobial activity UV–Visible spectroscopy Colony forming units TEM 



Silver nanoparticles


Zone of inhibition


Colony forming units



The authors acknowledge the financial support (Grant No. ASP/01/RE/MED/2016/42) and laboratory facilities provided by the University of Sri Jayewardenepura.


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Copyright information

© Association of Microbiologists of India 2018

Authors and Affiliations

  • M. M. K. Peiris
    • 1
  • S. S. N. Fernando
    • 1
  • P. M. Jayaweera
    • 2
  • N. D. H. Arachchi
    • 2
  • T. D. C. P. Guansekara
    • 1
  1. 1.Department of Microbiology, Faculty of Medical SciencesUniversity of Sri JayewardenepuraNugegodaSri Lanka
  2. 2.Department of Chemistry, Faculty of Applied SciencesUniversity of Sri JayewardenepuraNugegodaSri Lanka

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