Potential of Marine-Derived Fungi to Remove Hexavalent Chromium Pollutant from Culture Broth
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Chromium (Cr) released from industrial units such as tanneries, textile and electroplating industries is detrimental to the surrounding ecosystems and human health. The focus of the present study was to check the Cr(VI) removal efficiency by marine-derived fungi from liquid broth. Amongst the three Cr(VI) tolerant isolates, #NIOSN-SK56-S19 (Aspergillus sydowii) showed Cr-removal efficiency of 0.01 mg Cr mg−1 biomass resulting in 26% abatement of total Cr with just 2.8 mg of biomass produced during the growth in 300 ppm Cr(VI). Scanning Electron Microscopy revealed aggregation of mycelial biomass with exopolysaccharide, while Electron Dispersive Spectroscopy showed the presence of Cr2O3 inside the biomass indicating presence of active Cr(VI) removal mechanisms. This was further supported when the Cr(VI) removal was monitored using DPC (1,5-diphenylcarbazide) method. The results of this study point to the potential of marine-derived fungal isolates for Cr(VI) removal.
KeywordsBiosorption Halotolerant Heavy metal Hexavalent chromium Marine-derived fungi
The first author is thankful to Council of Scientific and Industrial Research (CSIR) for the fellowship (Ref No.: 18-12/2011(ii)EU-V). All the authors are thankful to Head, BOD and Director, CSIR-NIO for the facilities. The studies were funded through the Project PSC0206. The authors are thankful to Mr. Areef Sardar for carrying out SEM and EDS analysis. This work is part of the doctoral thesis to be submitted to Goa University in Department of Microbiology. The manuscript is NIO contribution No. 6186.
Compliance with Ethical Standards
Conflict of interest
The authors declare no financial or commercial conflict of interest.
- 5.Bishnoi NR, Garima (2005) Fungus- an alternative for bioremediation of heavy metal containing wastewater: a review. J Sci Ind Res India 64:93–100Google Scholar
- 9.Nazareth S, Gaitonde S, Marbaniang T (2012) Metal resistance of halotolerant fungi from mangroves and salterns of Goa, India. Kavaka 40:15–21Google Scholar
- 15.Akhtar S, Hassan MM, Ahmad R, Suthor V, Yasin M (2013) Metal tolerance potential of filamentous fungi isolated from soils irrigated with untreated municipal effluent. Soil Environ 32:55–62Google Scholar
- 16.White TJ, Bruns T, Lee S, Taylor J (1990) Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. In: Innis MA et al (eds) PCR protocols: a guide to methods and applications. Academic Press, New York. https://doi.org/10.1016/b978-0-12-372180-8.50042-1 Google Scholar
- 18.Tamura K, Peterson D, Peterson N, Stecher G, Nei M, Kumar S (2011) MEGA5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. Mol Biol Evol 28:2731–2739. https://doi.org/10.1093/molbev/msr121 CrossRefPubMedPubMedCentralGoogle Scholar
- 23.Raghukumar S, Raghukumar C, Manohar CS (2014) Fungi living in diverse extreme habitats of the marine environment. Kavaka 42:145–153Google Scholar
- 25.Nayak SS, Gonsalves V, Nazareth SW (2012) Isolation and salt tolerance of halophilic fungi from mangroves and solar salterns in Goa-India. Indian J Geomar Sci 41:164–172Google Scholar
- 27.Al-Abboud MA, Alawlaqi MM (2011) Biouptake of copper and their impact on fungal fatty acids. Aust J Basic Appl Sci 5:283–290Google Scholar
- 32.Faryal R, Lodhi A, Hameed A (2006) Isolation, Characterization and biosorption of Zinc by indigenous fungal strains Aspergillus fumigatus RH05 and Aspergillus flavus RH07. Pak J Bot 38:817–832Google Scholar
- 33.Abbas SH, Ismail IM, Mostafa TM, Sulaymon AH (2014) Biosorption of heavy metals : a review. J Chem Sci Technol 3:74–102Google Scholar
- 34.Merrie JS, Sheela R, Saswathi N, Prakasham RS, Ramakrishna SV (1998) Biosorption of chromium VI using Rhizopus arrhizus. Indian J Exp Biol 36:1052–1055Google Scholar
- 35.Jakubiak M, Giska I, Asztemborska M, Bystrzejewska-Piotrowska G (2014) Bioaccumulation and biosorption of inorganic nanoparticles: factors affecting the efficiency of nanoparticle mycoextraction by liquid- grown mycelia of Pleurotus eryngii and Trametes versicolor. Mycol Prog 13:525–532. https://doi.org/10.1007/s11557-013-0933-3 CrossRefGoogle Scholar
- 38.Annamalai K, Nair AM, Chinnaraju S, Kuppusamy S (2014) Chromium (III) nanoparticles synthesis using the biosorption and bioreduction with Bacillus subtilis: effect of pH and temperature. Int J ChemTech Res 6:1910–1912Google Scholar
- 39.Annamalai K, Nair AM, Chinnaraju S, Kuppusamy S (2014) Removal of chromium from contaminated effluent and simultaneously green nanoparticle synthesis using Bacillus subtilis. Malaya J Biosci 1:13–18Google Scholar
- 44.Baldrian P, Gabriel J (2003) Adsorption of heavy metal on microbial biomass: Use of biosorption for removal metals from metal solutions. In: Sasek V et al (eds) The utilization of bioremediation to reduce soil contamination: problems and solutions, vol 19. Springer, DordrechtGoogle Scholar