Indian Journal of Microbiology

, Volume 58, Issue 2, pp 146–158 | Cite as

In Vitro Anticancer Activity of Staphyloxanthin Pigment Extracted from Staphylococcus gallinarum KX912244, a Gut Microbe of Bombyx mori

  • Delicia Avilla Barretto
  • Shyam Kumar Vootla
Original Research Article


The present study reports the in vitro biological nature of the pigment produced by Staphylococcus gallinarum KX912244, isolated as the gut microflora bacterium of the insect Bombyx mori. The purified pigment was characterized as Staphyloxanthin based on bio-physical characterization techniques like Fourier transform infrared spectroscopy, high performance liquid chromatography, Proton nuclear magnetic resonance spectroscopy (1H NMR), Liquid chromatography-Mass spectroscopy and Gas chromatography-Mass spectroscopy. The Staphyloxanthin pigment presented considerable biological properties including in vitro antimicrobial activity against pathogens Staphylococcus aureus, Escherichia coli and Candida albicans; in vitro antioxidant activity by % DPPH free radical scavenging activity showing IC50 value of 54.22 µg/mL; DNA damage protection activity against reactive oxygen species and anticancer activity evaluated by cytotoxicity assay against 4 different cancer cell lines like the Dalton’s lymphoma ascites with IC50 value 6.20 ± 0.02 µg/mL, Ehrlich ascites carcinoma having IC50 value 6.48 ± 0.15 µg/mL, Adenocarcinomic human alveolar basal epithelial cells (A549 Lung carcinoma) bearing IC50 value 7.23 ± 0.11 µg/mL and Mus mucus skin melanoma (B16F10) showing IC50 value 6.58 ± 0.38 µg/mL and less cytotoxicity towards non-cancerous human fibroblast cell lines (NIH3T3) with IC50 value of 52.24 µg/mL. The present study results suggest that Staphyloxanthin acts as a potential therapeutic agent especially due to its anticancer property.


Cancer Staphyloxanthin Staphylococcus gallinarum Bombyx mori Free radicals 



Delicia Avilla Barretto is grateful to Department of Science & Technology- Innovation in Science Pursuit for Inspired Research (DST-INSPIRE) with (Award letter number: DST/INSPIRE FELLOWSHIP/2013/349 Dated: 16-08-2013) for financial assistance to complete this research work. The authors are thankful to the technical staff of University Scientific and Instruments Centre (USIC), Karnatak University Dharwad and National Collection of Industrial Microorganisms (NCIM)- National Chemical Laboratory (NCL), Pune and for their technical support.

Supplementary material

12088_2018_718_MOESM1_ESM.docx (754 kb)
Supplementary material 1 (DOCX 753 kb)


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Copyright information

© Association of Microbiologists of India 2018

Authors and Affiliations

  1. 1.Department of Biotechnology and MicrobiologyKarnatak UniversityDharwadIndia

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