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Indian Journal of Microbiology

, Volume 58, Issue 2, pp 201–207 | Cite as

Incubation of Aquilaria subintegra with Microbial Culture Supernatants Enhances Production of Volatile Compounds and Improves Quality of Agarwood Oil

  • Sakon Monggoot
  • Chadin Kulsing
  • Yong Foo Wong
  • Patcharee Pripdeevech
Original Research Article

Abstract

Incubation with microbial culture supernatants improved essential oil yield from Aquilaria subintegra woodchips. The harvested woodchips were incubated with de man, rogosa and sharpe (MRS) agar, yeast mold (YM) agar medium and six different microbial culture supernatants obtained from Lactobacillus bulgaricus, L. acidophilus, Streptococcus thermophilus, Lactococcus lactis, Saccharomyces carlsbergensis and S. cerevisiae prior to hydrodistillation. Incubation with lactic acid bacteria supernatants provided higher yield of agarwood oil (0.45% w/w) than that obtained from yeast (0.25% w/w), agar media (0.23% w/w) and water (0.22% w/w). The composition of agarwood oil from all media and microbial supernatant incubations was investigated by using gas chromatography-mass spectrometry. Overall, three major volatile profiles were obtained, which corresponded to water soaking (control), as well as, both YM and MRS media, lactic acid bacteria, and yeast supernatant incubations. Sesquiterpenes and their oxygenated derivatives were key components of agarwood oil. Fifty-two volatile components were tentatively identified in all samples. Beta-agarofuran, α-eudesmol, karanone, α-agarofuran and agarospirol were major components present in most of the incubated samples, while S. cerevisiae-incubated A. subintegra provided higher amount of phenyl acetaldehyde. Microbial culture supernatant incubation numerically provided the highest yield of agarwood oil compared to water soaking traditional method, possibly resulting from activity of extracellular enzymes produced by the microbes. Incubation of agarwood with lactic acid bacteria supernatant significantly enhanced oil yields without changing volatile profile/composition of agarwood essential oil, thus this is a promising method for future use.

Keywords

Aquilaria subintegra Agarwood oil Culture supernatant GC–MS Lactic acid bacteria 

Notes

Acknowledgements

The authors gratefully acknowledge support provided by the Mae Fah Luang University for funding. We are grateful to personnel of the Queen Sirikit Botanical Garden for their help in collection and identification of A. subintegra plants and the Scientific and Technological Instrument Centre of Mae Fah Luang University for their instrumental support concerning the GC–MS.

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Copyright information

© Association of Microbiologists of India 2018

Authors and Affiliations

  • Sakon Monggoot
    • 1
  • Chadin Kulsing
    • 2
  • Yong Foo Wong
    • 3
  • Patcharee Pripdeevech
    • 1
    • 4
  1. 1.School of ScienceMae Fah Luang UniversityChiang RaiThailand
  2. 2.Department of Chemistry, Faculty of ScienceChulalongkorn UniversityBangkokThailand
  3. 3.School of ChemistryMonash UniversityMelbourneAustralia
  4. 4.Center of Chemical Innovation for Sustainability (CIS)Mae Fah Luang UniversityChiang RaiThailand

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