Indian Journal of Microbiology

, Volume 58, Issue 2, pp 234–238 | Cite as

Psychrotolerant Sphingobacterium kitahiroshimense LT-2 Isolated from Dhundi Glacier, Himachal Pradesh: Origin Prediction and Future Application

  • Shivika Sharma
  • Subhankar Chatterjee
Short Communication


A psychrotolerant bacterium, isolated from Dhundi Glacier, Himachal Pradesh (India) was identified as Sphingobacterium kitahiroshimense LT-2 on the basis of biochemical, molecular and phylogenetic analysis. Sphingobacterium kitahiroshimense was first reported from Japan and was isolated from the city of Kitahiroshima, Hokkaido, Japan. In this report we have discussed about the origin of our strain and predicted that air masses and dust associated microbial cells transportation phenomena may be applicable for the origin of this species in this region. Enzymes and secondary metabolites secreted by the genus Sphingobacterium have enormous potentiality regarding their biotechnological application. Preliminary study of our strain based on metabolic profiling through HPLC showed many new metabolites were secreted by the bacterium when grown in presence of different sugar medium at 28 °C. As far as our knowledge this is the first report about Sphingobacterium species isolated from this region. This preliminary finding will help to draw an idea about the bacterial population in this Himalayan Glaciers (in HP) as well as biotechnological application of this strain can be explored further.


Psychrophiles Psychrotolerant bacteria Sphingobacterium Secondary metabolites Cold active enzymes Dust associated microbial cells transportation 



Financial support for this work was provided by SERB-DST, Govt. of India (PDF/2016/000818). We are thankful to Ms. Riju Parmar and Dr. Anurag Linda for their help in strain collection. We gratefully acknowledged IMTECH, Chandigarh for their support regarding the identification of the strain.

Compliance with Ethical Standards

Conflict of interest

Authors declare no conflict of interest.

Supplementary material

12088_2018_712_MOESM1_ESM.tif (166 kb)
Supplementary Fig. 1 (S1): HPLC profile of secreted secondary metabolites of Sphingobacterium kitahiroshimense LT-2 grown in presence of Glucose at 28 °C. Peaks exclusively appeared in the Glucose medium have also been shown. The secondary metabolite profiling was carried out on UltiMate 3000 UHPLC+ system, Thermo Scientific; The HPLC system was equipped with HYPERSIL GOLD C18 reverse phase column (250 mm × 4.6 mm; particle size: 5µ, Thermo Scientific). The analyte was eluted in multistep gradient modes using mobile phase of methanol (A) and water (B): 0–2 min 0–60%A; 2–30 min from 60% A to 90%A; 30–40 min 90%A; 40–45 min 90% A to 60% A; 45–50 min 60% A to 0% A at a flow rate of 0.8 ml/min. This was followed by washing and re-equilibration steps. A quality control sample was injected at regular interval to monitor the stability of the method. The analyte was detected using Diode Array Detector at 270 nm wavelengths. The data so generated was analyzed by using Chromeleon software. (TIFF 165 kb)


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Copyright information

© Association of Microbiologists of India 2018

Authors and Affiliations

  1. 1.Bioremediation and Metabolomics Research Group, Department of Chemistry and Chemical Sciences, School of Physical and Material SciencesCentral University of Himachal PradeshKangraIndia
  2. 2.Department of Environmental Sciences, School of Earth and Environmental SciencesCentral University of Himachal PradeshKangraIndia

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