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Indian Journal of Microbiology

, Volume 58, Issue 2, pp 214–221 | Cite as

Extracellular Synthesis and Characterization of Gold Nanoparticles Using Mycobacterium sp. BRS2A-AR2 Isolated from the Aerial Roots of the Ghanaian Mangrove Plant, Rhizophora racemosa

  • Mustafa Camas
  • Anil Sazak Camas
  • Kwaku Kyeremeh
Original Research Article

Abstract

Through the use of genomes that have undergone millions of years of evolution, marine Actinobacteria are known to have adapted to rapidly changing environmental pressures. The result is a huge chemical and biological diversity among marine Actinobacteria. It is gradually becoming a known fact that, marine Actinobacteria have the capability to produce nanoparticles which have reasonable sizes and structures with possible applications in biotechnology and pharmacology. Mycobacterium sp. BRS2A-AR2 was isolated from the aerial roots of the mangrove plant Rhizophora racemosa. The Mycobacterium was demonstrated for the first time ever to produce AuNPs with sizes that range between 5 and 55 nm. The highest level absorbance of the biosynthesized AuNPs was typical for actinobacterial strains (2.881 at 545 nm). The polydispersity index was measured as 0.207 in DLS and the zeta potential was negatively charged (− 28.3 mV). Significant vibration stretches were seen at 3314, 2358, 1635 and 667 cm−1 in FT-IR spectra. This demonstrated the possible use of small aliphatic compounds containing –COOH, –OH, –Cl and –NH2 functional groups in the stabilization of the AuNPs. The effect of the biosynthesized AuNPs on HUVEC and HeLA cell lines was measured at 48 h. IC50 values were determined at 3500 µg/ml concentration for HUVEC and HeLA cell lines at 45.25 and 53.41% respectively.

Keywords

Actinobacteria Gold nanoparticles Cytotoxicity Spectroscopy 

Notes

Acknowledgements

KK wishes to thank Centre for African Wetlands (CAW), University of Ghana for providing seed funding to enable collection of samples. KK is grateful to Cambridge-Africa Partnership for Research Excellence (CAPREx)—funded by the Carnegie Corporation of New York, for a Postdoctoral Fellowship. KK also appreciates Cambridge-Africa ALBORADA Research Fund for support.

Supplementary material

12088_2018_710_MOESM1_ESM.docx (17 kb)
Supplementary material 1 (DOCX 17 kb)

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Copyright information

© Association of Microbiologists of India 2018

Authors and Affiliations

  1. 1.Department of BioengineeringMunzur UniversityTunceliTurkey
  2. 2.Department of Chemistry, School of Physical and Mathematical SciencesUniversity of GhanaLegon-AccraGhana

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