Indian Journal of Microbiology

, Volume 59, Issue 2, pp 154–160 | Cite as

Co-digestion of Biowastes to Enhance Biological Hydrogen Process by Defined Mixed Bacterial Cultures

  • Sanjay K. S. Patel
  • Subhasree Ray
  • Jyotsana Prakash
  • Ji Hyang Wee
  • Sang-Yong Kim
  • Jung-Kul LeeEmail author
  • Vipin Chandra KaliaEmail author
Original Research Article


Co-digestion of biowastes for hydrogen (H2) production using defined mixed cultures can overcome the high risk of failure due to contamination and imbalanced nutrient status. H2 production from biowastes—pea-shells, potato peels (PP), onion peels (OP) and apple pomace, either individually or in various combinations was evaluated by hydrolyzing with defined hydrolytic mixed bacterial culture (MHC5) and subjecting the hydrolysate to mixture of defined H2 producers (MMC6). Co-digestion of OP and PP hydrolysate supplemented at H2 production stage with GM-2 and M-9 media resulted in 95 and 102 l H2/kg of Total solids (TS), respectively compared to 84 l H2/kg of TS in control. Upscaling the process by digesting 4.0 l slurry (16-fold) resulted in 88.5 and 95 l H2/kg of TS, respectively compared to 72 l H2/kg of TS in control. Thus, H2 production by co-digestion of biowastes could be improved through the supplementation with very dilute medium (0.1 ×) and selection of suitable biowastes under unsterile conditions. The overall efficiency can be further enhanced by integrating it with bioprocesses for biopolymers such as polyhydroxyalkanoates and or biofuels like methane production.


Biowaste Bacillus Hydrogen Onion peels Potato peels Mixed microbial culture 



This research was supported by Basic Science Research Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Science, ICT and Future Planning (NRF-2018H1D3A2001746, 2013M3A6A8073184). This research was also supported by KU Research Professor Program of Konkuk University.

Compliance with Ethical Standards

Conflict of interest

The authors declare no conflict of interest.


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Copyright information

© Association of Microbiologists of India 2019

Authors and Affiliations

  1. 1.Department of Chemical EngineeringKonkuk UniversitySeoulRepublic of Korea
  2. 2.Department of Microbial Biotechnology and GenomicsCSIR-Institute of Genomics and Integrative BiologyDelhiIndia
  3. 3.Department of Food Science and BiotechnologyShin-Ansan UniversityAnsanRepublic of Korea

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