In Vitro Antibacterial Activity of Phlorotannins from Edible Brown Algae, Eisenia bicyclis Against Streptomycin-Resistant Listeria monocytogenes

Abstract

Listeria monocytogenes (LM) is an important food borne pathogen responsible for listeriosis. Further, LM is an etiological agent associated with life threatening conditions like meningitis and encephalitis. Biofilm forming and drug resistant LM may potentially become difficult to treat infections and hence effective controlling measures are required to prevent LM infections. In view of this, the present study evaluated an anti-listerial potential of edible brown seaweed, Eisenia bicyclis, by disc diffusion and micro-dilution methods. The results of the present study suggested that the anti-listerial activity of various phlorotannins isolated form E. bicyclis were in the range of 16–256 µg/ml. Among the phlorotannins isolated, fucofuroeckol-A (FAA) exhibited the highest anti-listerial potential (MIC range 16–32 µg/ml) against LM strains tested. Further, in checker board synergy assays, FFA-streptomycin combination exhibited significant synergy (fractional inhibitory concentration index, ∑FIC < 0.5) against aminoglycoside resistant clinical strains of LM. The results of the present study suggested the potential use of edible seaweed E. bicyclis as a source of natural phlorotannins to control food borne pathogenic infections.

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Acknowledgements

This research was supported by Marine Biotechnology Program (20150220) funded by Ministry of Oceans and Fisheries, Republic of Korea. The pathogens for this study were provided by the Gyeongsang National University Hospital Branch of National Culture Collection for Pathogens (GNUH-NCCP).

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Correspondence to Young-Mog Kim.

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Kim, HJ., Dasagrandhi, C., Kim, SH. et al. In Vitro Antibacterial Activity of Phlorotannins from Edible Brown Algae, Eisenia bicyclis Against Streptomycin-Resistant Listeria monocytogenes . Indian J Microbiol 58, 105–108 (2018). https://doi.org/10.1007/s12088-017-0693-x

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Keywords

  • Antibiotic resistance
  • Anti-listerial agents
  • Eisenia bicyclis
  • Phlorotannins