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7,10-Epoxyoctadeca-7,9-dienoic Acid: A Small Molecule Adjuvant That Potentiates β-Lactam Antibiotics Against Multidrug-Resistant Staphylococcus aureus

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Abstract

The emergence of methicillin-resistant Staphylococcus aureus (MRSA) infections with multi-drug resistance needs effective and alternative control strategies. In this study we investigated the adjuvant effect of a novel furan fatty acid, 7,10-epoxyoctadeca-7,9-dienoic acid (7,10-EODA) against multidrug-resistant S. aureus (MDRSA) strain 01ST001 by disc diffusion, checker board and time kill assays. Further the membrane targeting action of 7,10-EODA was investigated by spectroscopic and confocal microscopic studies. 7,10-EODA exerted synergistic activity along with β-lactam antibiotics against all clinical MRSA strains, with a mean fractional inhibitory concentration index below 0.5. In time-kill kinetic study, combination of 7,10-EODA with oxacillin, ampicillin, and penicillin resulted in 3.8–4.2 log10 reduction in the viable counts of MDRSA 01ST001. Further, 7,10-EODA dose dependently altered the membrane integrity (p < 0.001) and increased the binding of fluorescent analog of penicillin, Bocillin-FL to the MDRSA cells. The membrane action of 7,10-EODA further facilitated the uptake of several other antibiotics in MDRSA. The results of the present study suggested that 7,10-EODA could be a novel antibiotic adjuvant, especially useful in repurposing β-lactam antibiotics against multidrug-resistant MRSA.

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Acknowledgements

This research was supported by the Basic Science Research Program of the National Research Foundation of Korea (NRF) funded by the Ministry of Science, ICT, and future planning (2015R1A2A2A01005656).

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Authors and Affiliations

  1. School of Food Science and Biotechnology, Kyungpook National University, Daegu, 702-701, South Korea

    Chakradhar Dasagrandhi & Hak-Ryul Kim

  2. Department of Food and Nutrition, Korea University, Seoul, South Korea

    Young-Soon Kim & In-Hwan Kim

  3. Renewable Product Technology Research Unit, National Center for Agricultural Utilization Research, ARS, USDA, Peoria, IL, USA

    Ching T. Hou

  4. Institute of Agricultural Science and Technology, Kyungpook National University, Daegu, South Korea

    Hak-Ryul Kim

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  1. Chakradhar Dasagrandhi
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  2. Young-Soon Kim
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Correspondence to Hak-Ryul Kim.

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Dasagrandhi, C., Kim, YS., Kim, IH. et al. 7,10-Epoxyoctadeca-7,9-dienoic Acid: A Small Molecule Adjuvant That Potentiates β-Lactam Antibiotics Against Multidrug-Resistant Staphylococcus aureus . Indian J Microbiol 57, 461–469 (2017). https://doi.org/10.1007/s12088-017-0680-2

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  • DOI: https://doi.org/10.1007/s12088-017-0680-2

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