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Ultra-High Efficient Colony PCR for High Throughput Screening of Bacterial Genes

Abstract

Current colony PCR methods are not suitable for screening genes encoded in genomic DNA and are limited to E. coli host strains. Here, we describe an ultra-high efficient colony PCR method for high throughput screening of bacterial genes embedded in the genomic DNA of any bacterial species. This new technique expands colony PCR method to several hosts as well as offers a rapid, less expensive and reliable bacterial genomic DNA extraction.

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Acknowledgements

This research was financially supported by the “Global accompanied growth R&BD program (N042600010)” through the Ministry of Trade, Industry and Energy (MOTIE, Korea).

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Correspondence to Seungkoo Lee.

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Jamal, M.A.H.M., Sharma, S.P., Chung, HJ. et al. Ultra-High Efficient Colony PCR for High Throughput Screening of Bacterial Genes. Indian J Microbiol 57, 365–369 (2017). https://doi.org/10.1007/s12088-017-0665-1

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  • DOI: https://doi.org/10.1007/s12088-017-0665-1

Keywords

  • Colony PCR
  • DNA extraction
  • High throughput screening
  • Sonication
  • Sequencing