Skip to main content
Springer Nature Link
Log in

Cellulolytic Activity of Thermophilic Bacilli Isolated from Tattapani Hot Spring Sediment in North West Himalayas

  • Short Communication
  • Published:
Indian Journal of Microbiology Aims and scope Submit manuscript

Abstract

Eight thermophilic bacterial strains were isolated from Tattapani Hot spring and screened for various hydrolytic enzymes including cellulases. The isolated bacterial strains were identified as Geobacillus thermodenitrificans IP_WH1(KP842609), Bacillus licheniformis IP_WH2(KP842610), B. aerius IP_WH3(KP842611), B. licheniformis IP_WH4(KP842612), B. licheniformis IP_60Y(KP842613), G. thermodenitrificans IP_60A1(KP842614), Geobacillus sp. IP_60A2(KP842615) and Geobacillus sp. IP_80TP(KP842616) after 16S ribotying. Out of the eight isolates Geobacillus sp. IP_80TP grew best at 80 °C whereas rest of the isolates showed optimal growth at 60 °C. G. thermodenitrificans IP_WH1 produced a thermotolerant cellulase with maximum activity at 60 °C.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Subscribe and save

Springer+ Basic
$34.99 /Month
  • Get 10 units per month
  • Download Article/Chapter or eBook
  • 1 Unit = 1 Article or 1 Chapter
  • Cancel anytime
Subscribe now

Buy Now

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3

Explore related subjects

Discover the latest articles and news from researchers in related subjects, suggested using machine learning.

References

  1. Takai K, Nakamura K, Toki T, Tsunogai U, Miyazaki M, Miyazaki J, Hirayama H, Nakagawa S, Nunoura T, Horikoshi K (2008) Cell proliferation at 122 °C and isotopically heavy CH4 production by a hyperthermophilic methanogen under high-pressure cultivation. Proc Natl Acad Sci 105:10949–10954. doi:10.1073/pnas.0712334105

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  2. Kuhad RC, Gupta R, Singh A (2011) Microbial cellulases and their industrial applications. Enzyme Res, Article ID 280696. doi:10.4061/2011/280696

  3. Zamost BL, Nielsen HK, Starnes RL (1991) Thermostable enzymes for industrial applications. J Ind Microbiol 8:71–81. doi:10.1007/BF01578757

    Article  CAS  Google Scholar 

  4. Cummins KW (1962) An evaluation of some techniques for the collection and analysis of benthic samples with special emphasis on lotic waters. Am Midl Nat 67:477–504

    Article  Google Scholar 

  5. Adiguzel A, Inan K, Sahin F, Arasoglu T, Gulluce M (2011) Molecular diversity of thermophilic bacteria isolated from Pasinler hot spring (Erzurum, Turkey). Turk J Biol 35:267–274. doi:10.3906/biy-0908-7

    CAS  Google Scholar 

  6. Aanniz T, Ouadghiri M, Melloul M, Swings J, Elfahime E, Ibijbijen J, Ismaili M, Amar M (2015) Thermophilic bacteria in Moroccan hot springs, salt marshes and desert soils. Braz J Microbiol 46:443–453. doi:10.1590/S1517-838246220140219

    Article  PubMed  PubMed Central  Google Scholar 

  7. Kumar B, Trivedi P, Mishra AK, Pandey A, Palni LMS (2004) Microbial diversity of soil from two hot springs in Uttaranchal Himalaya. Microbiol Res 159:141–146. doi:10.1016/j.micres.2004.01.004

    Article  CAS  PubMed  Google Scholar 

  8. Piterina AV, Bartlett J, Pembroke JT (2010) Molecular analysis of bacterial community DNA in sludge undergoing autothermal thermophilic aerobic digestion (ATAD): pitfalls and improved methodology to enhance diversity recovery. Diversity 2:505–526. doi:10.3390/d204050

    Article  CAS  Google Scholar 

  9. Tamura K, Stecher G, Peterson D, Filipski A, Kumar S (2013) MEGA6: molecular evolutionary genetics analysis version 6.0. Mol Biol Evol 30:2725–2729. doi:10.1093/molbev/mst197

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  10. Chauhan A, Chauhan G, Gupta PC, Goyal P, Kaushik P (2010) In vitro antibacterial evaluation of Anabaena sp. against several clinically significant microflora and HPTLC analysis of its active crude extracts. Indian J pharmacol 42:105–107. doi:10.4103/0253-7613.64490

    Article  PubMed  PubMed Central  Google Scholar 

  11. Miller GL (1959) Use of dinitrosalicylic acid reagent for determination of reducing sugar. Anal Chem 31:426–428. doi:10.1021/ac60147a03

    Article  CAS  Google Scholar 

  12. Padilha IQM, Carvalho LCT, Dias PVS, Grisi TCSL, Silva FLH, Santos SFM, Araujo DAM (2015) Production and characterization of thermophilic carboxymethyl cellulase synthesized by Bacillus sp. Growing on sugarcane bagasse in submerged fermentation. Braz J Chem Eng 32:35–42. doi:10.1590/0104-6632.20150321s00003298

    Article  CAS  Google Scholar 

  13. Ladeira SA, Cruz E, Delatorre AB, Barbosa JB, Martins MLL (2015) Cellulase production by thermophilic Bacillus sp. SMIA-2 and its detergent compatibility. Electron J Biotechnol 18:110–115. doi:10.1016/j.ejbt.2014.12.008

    Article  CAS  Google Scholar 

  14. Christopher LP, Zambare VP, Zambare A, Kumar H, Malek L (2015) A thermo-alkaline lipase from a new thermophile Geobacillus thermodenitrificans AV-5 with potential application in biodiesel production. J Chem Technol Biotechnol 90:2007–2016. doi:10.1002/jctb.4678

    Article  CAS  Google Scholar 

  15. Ezeji TC, Wolf A, Bahl H (2005) Isolation, characterization, and identification of Geobacillus thermodenitrificans HRO10, an alpha-amylase and alpha-glucosidase producing thermophile. Can J Microbiol 51:685–693

    Article  CAS  PubMed  Google Scholar 

  16. Rastogi G, Bhalla A, Adhikari A, Bischoff KM, Hughes SR, Christopher LP, Sani RK (2010) Characterization of thermostable cellulases produced by Bacillus and Geobacillus strains. Bioresour Technol 101:8798–8806. doi:10.1016/j.biortech.2010.06.001

    Article  CAS  PubMed  Google Scholar 

  17. Mawadza C, Hatti-Kaul R, Zvauya R, Mattiasson B (2000) Purification and characterization of cellulases produced by two Bacillus strains. J Biotechnol 83:177–187

    Article  CAS  PubMed  Google Scholar 

  18. Tai SK, Lin HPP, Kuo J, Liu JK (2004) Isolation and characterization of a cellulolytic Geobacillus thermoleovorans T4 strain from sugar refinery wastewater. Extremophiles 8:345–349. doi:10.1007/s00792-004-0395-2

    Article  CAS  PubMed  Google Scholar 

Download references

Acknowledgments

The work was conducted by the financial support provided under the UGC-SAP programme.

Author information

Author notes

    Authors and Affiliations

    1. School of Biotechnology, University of Jammu, Jammu, J&K, 180006, India

      Indu Priya, M. K. Dhar, B. K. Bajaj, Sanjana Koul & Jyoti Vakhlu

    Authors
    1. Indu Priya
      View author publications

      Search author on:PubMed Google Scholar

    2. M. K. Dhar
      View author publications

      Search author on:PubMed Google Scholar

    3. B. K. Bajaj
      View author publications

      Search author on:PubMed Google Scholar

    4. Sanjana Koul
      View author publications

      Search author on:PubMed Google Scholar

    5. Jyoti Vakhlu
      View author publications

      Search author on:PubMed Google Scholar

    Corresponding author

    Correspondence to Jyoti Vakhlu.

    Additional information

    M. K. Dhar, B. K. Bajaj and Sanjana Koul have contributed equally to this work.

    Electronic supplementary material

    Below is the link to the electronic supplementary material.

    Supplementary material 1 (DOC 60 kb)

    Rights and permissions

    Reprints and permissions

    About this article

    Check for updates. Verify currency and authenticity via CrossMark

    Cite this article

    Priya, I., Dhar, M.K., Bajaj, B.K. et al. Cellulolytic Activity of Thermophilic Bacilli Isolated from Tattapani Hot Spring Sediment in North West Himalayas. Indian J Microbiol 56, 228–231 (2016). https://doi.org/10.1007/s12088-016-0578-4

    Download citation

    • Received:

    • Accepted:

    • Published:

    • Issue Date:

    • DOI: https://doi.org/10.1007/s12088-016-0578-4

    Keywords