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Development of PCR Diagnostic System for Detection of the Seed-Transmitted Tobacco Ringspot Virus in Quarantine

Indian Journal of Microbiology volume 55, pages 231–233 (2015)Cite this article

Abstract

Tobacco ringspot virus (TRSV) is a plant quarantine virus in Korea. As such, a TRSV examination is conducted when importing various crops. In this study, RT-PCR and nested PCR systems for TRSV detection in quarantine sites, and the modified-positive control plasmid for proving laboratory contamination and false positive reactions were developed. The developed diagnostic system was used to detect TRSV in the quarantine site. It revealed that from 2012 to August 2014, a total of 12 cases were detected in imported various crops. The system is expected to continue contributing to TRSV detection in plant quarantine.

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Acknowledgments

The present research was conducted with the support of Animal and Plant Quarantine Agency in 2009–2011.

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    Affiliations

    1. Environmental Infrastructure Research Department, National Institute of Environmental Research, Incheon, 440-170, Korea

      S. Lee, G. Lee & I. C. Choi

    2. Department of Microbiology, College of Natural Sciences, Dankook University, Cheonan, 330-714, Korea

      J. Y. Rho

    Authors
    1. S. Lee
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    2. G. Lee
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    3. I. C. Choi
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    4. J. Y. Rho
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    Corresponding author

    Correspondence to J. Y. Rho.

    Additional information

    S. Lee and I. C. Choi have equally contributed to this work.

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    Lee, S., Lee, G., Choi, I.C. et al. Development of PCR Diagnostic System for Detection of the Seed-Transmitted Tobacco Ringspot Virus in Quarantine. Indian J Microbiol 55, 231–233 (2015). https://doi.org/10.1007/s12088-015-0518-8

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    • DOI: https://doi.org/10.1007/s12088-015-0518-8

    Keywords

    • Tobacco ringspot virus
    • TRSV
    • RT-PCR
    • Nested PCR
    • Quarantine