Abstract
Haloarchaea are predominant in the salt crystallizers of the Rann of Kutch when the concentration of salts approaches saturation levels. The obligate and extreme halophilic archaeon 3A1-DGR, isolated from a salt crystallizer pond of the Little Rann of Kutch, India, needs minimum of 10 % NaCl in the growth medium. To understand the mechanism(s) of osmotolerance and adaptation at extreme osmolarity, and to mine relevant gene(s), the genome of this haloarchaeon, 3A1-DGR, was sequenced. We report here, the 2.88 Mb draft genome sequence of the haloarchaeon 3A1-DGR, with G+C content of 68 % and the possible involvement of 43 genes in stress tolerance. Further studies of the genome of this haloarchaeon would be required to identify gene(s) that might be responsible for imparting extreme osmotolerance.
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Acknowledgments
The work was carried out in the subproject “Exploring bacterial and archaebacterial diversity in Kutch eco-region of Gujarat for agricultural and industrial applications” under the Application of Microorganisms in Agriculture and Allied Sectors (AMAAS) of the Indian Council of Agricultural Research (ICAR). Authors thank ICAR for funding through AMAAS. Authors thank the Directors of the Directorate of Groundnut Research, Junagadh, and NBAIM, Mau, for help during the course of this study. The authors thank Macrogen Inc., South Korea, and Sequencher Tech Pvt. Ltd., Ahmedabad, India, for the genomic services.
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Pal, K.K., Dey, R., Thomas, M. et al. Draft Genome Sequence of an Extreme Haloarchaeon 3A1-DGR Isolated from a Saltern Crystallizer of the Little Rann of Kutch, India. Indian J Microbiol 54, 471–473 (2014). https://doi.org/10.1007/s12088-014-0483-7
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DOI: https://doi.org/10.1007/s12088-014-0483-7