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Indian Journal of Microbiology

, Volume 54, Issue 4, pp 413–418 | Cite as

Plant Growth Promoting Bacteria from Cow Dung Based Biodynamic Preparations

  • T. K. Radha
  • D. L. N. Rao
Original Article

Abstract

Indigenous formulations based on cow dung fermentation are commonly used in organic farming. Three biodynamic preparations viz., Panchagavya (PG), BD500 and ‘Cow pat pit’ (CPP) showed high counts of lactobacilli (109 ml−1) and yeasts (104 ml−1). Actinomycetes were present only in CPP (104 ml−1) and absent in the other two. Seven bacterial isolates from these ferments were identified by a polyphasic approach: Bacillus safensis (PG1), Bacillus cereus (PG2, PG4 PG5), Bacillus subtilis (BD2) Lysinibacillus xylanilyticus (BD3) and Bacillus licheniformis (CPP1). This is the first report of L. xylanilyticus and B. licheniformis in biodynamic preparations. Only three carbon sources—dextrose, sucrose and trehalose out of 21 tested were utilized by all the bacteria. None could utilize arabinose, dulcitol, galactose, inositol, inulin, melibiose, raffinose, rhamnose and sorbitol. All the strains produced indole acetic acid (1.8–3.7 μg ml−1 culture filtrate) and ammonia. None could fix nitrogen; but all except B. safensis and B. licheniformis could solubilize phosphorous from insoluble tri-calcium phosphate. All the strains except L. xylaniliticus exhibited antagonism to the plant pathogen Rhizoctonia bataticola whereas none could inhibit Sclerotium rolfsi. In green house experiment in soil microcosms, bacterial inoculation significantly promoted growth of maize; plant dry weight increased by ~21 % due to inoculation with B. cereus (PG2). Results provide a basis for understanding the beneficial effects of biodynamic preparations and industrial deployment of the strains.

Keywords

Bacillus spp. Biofertilizer BD500 Cow pat pit Fermentation Lysinibacillus sp. Panchagavya 

Notes

Acknowledgments

We thank Dr. P. Ramesh, Sr. Scientist, Network Project on Organic Farming, IISS, Bhopal for supplying the biodynamic preparations.

Supplementary material

12088_2014_468_MOESM1_ESM.docx (15 kb)
Supplementary material 1 (DOCX 15 kb)

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Copyright information

© Association of Microbiologists of India 2014

Authors and Affiliations

  1. 1.Indian Institute of Soil ScienceBhopalIndia

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