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Efficacy of Cryptdin-2 as an Adjunct to Antibiotics from Various Generations Against Salmonella

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Abstract

Emerging drug resistance in Salmonella coupled with the recent poor success rate of antibiotic discovery programs of the pharmaceutical industry is a cause for significant concern. It has forced the scientific community to look for alternative new classes of antimicrobial compounds. In this context, combinations of antimicrobial peptides (AMPs) and conventional antibiotics have gained interest owing to their versatile applications. The present study was therefore planned to evaluate the synergistic effects, if any, of cryptdin-2, a mouse Paneth cell alpha-defensin, in combination with four different antibiotics i.e. ciprofloxacin, ceftriaxone, cefotaxime and chloramphenicol, which are conventionally used against Salmonella. Minimum bactericidal concentrations of the selected antimicrobial agents were determined by micro and macro broth dilution assays. In-vitro synergy between the agents was evaluated by fractional bactericidal concentration index (checkerboard test) and time-kill assay. Cryptdin-2-ciprofloxacin, cryptdin-2-ceftriaxone and cryptdin-2-cefotaxime combinations were found synergistic as evident by in vitro assays. This synergism provides an additional therapeutic choice by allowing the use of conventional antibiotics in conjunction with AMPs against MDR Salmonella.

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Acknowledgments

The authors express their gratitude to Mr. Navtej Singh at Central Instrumentation Laboratory, Panjab University, Chandigarh, India, for providing assistance in photographic analysis of the samples. The authors also wish to acknowledge the Council of Scientific and Industrial Research, New Delhi, for providing financial assistance to carry out this work.

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Authors and Affiliations

  1. Department of Microbiology, Basic Medical Sciences Block, Panjab University, Sector-14, Chandigarh, 160014, India

    Aman Preet Singh, Vijay Prabha & Praveen Rishi

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  1. Aman Preet Singh
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  2. Vijay Prabha
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  3. Praveen Rishi
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Correspondence to Praveen Rishi.

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Singh, A.P., Prabha, V. & Rishi, P. Efficacy of Cryptdin-2 as an Adjunct to Antibiotics from Various Generations Against Salmonella . Indian J Microbiol 54, 323–328 (2014). https://doi.org/10.1007/s12088-014-0463-y

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  • DOI: https://doi.org/10.1007/s12088-014-0463-y

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