Abstract
The aim of the study was to compare the preservative effectiveness of plant extracts (Matricaria chamomilla, Aloe vera, Calendula officinalis) and essential oils (Lavandulla officinalis, Melaleuca alternifolia, Cinnamomum zeylanicum) with methylparaben in cosmetic emulsions against skin microflora during 2 months of application by volunteers. Cosmetic emulsions with extracts (2.5 %), essential oils (2.5 %), methylparaben (0.4 %) or placebo were tested by 40 volunteers during 2 months of treatment. In order to determine microbial purity of the emulsions, the samples were taken after 0, 2, 4, 6 and 8 weeks of application. Throughout the trial period it was revealed that only cinnamon oil completely inhibited the growth of bacteria, yeast and mould, as compared to all other essential oils, plant extracts and methylparaben in the tested emulsions. This result shows that cinnamon oil could successfully replace the use of methylparaben in cosmetics, at the same time ensuring microbiological purity of a cosmetic product under its in-use and storage conditions.
References
Herman A, Herman AP, Domagalska BW, Młynarczyk A (2013) Essential oils and herbal extracts as antimicrobial agents in cosmetic emulsion. Indian J Microbiol 53:232–237. doi:10.1007/s12088-012-0329-0
Inouye S, Takizawa T, Yamaguchi H (2001) Antibacterial activity of essential oils and their major constituents against respiratory tract pathogens by gaseous contact. J Antimicrob Chemother 47:565–573. doi:10.1093/jac/47.5.565
Filoche SK, Soma K, Sissons CH (2005) Antimicrobial effects of essential oils in combination with chlorhexidine digluconate. Oral Microbiol Immunol 20:221–225. doi:10.1111/j.1399-302X.2005.00216.x
Bouhdid S, Abrini J, Amensour M, Zhiri A, Espuny MJ, Manresa A (2010) Functional and ultrastructural changes in Pseudomonas aeruginosa and Staphylococcus aureus cells induced by Cinnamomum verum essential oil. J Appl Microbiol 109:1139–1149. doi:10.1111/j.1365-2672.2010.04740.x
Herman A, Bochenek J, Herman AP (2013) Effect of cinnamon and lavender oils on FtsZ gene expression in the Staphylococcus aureus ATCC 29213. Appl Biochem Microbiol 49:481–484. doi:10.1134/S0003683813050049
Zani F, Minutello A, Maggi L, Santi P, Mazza P (1997) Evaluation of preservative effectiveness in pharmaceutical products: the use of a wild strain of Pseudomonas cepacia. J Appl Microbiol 83:322–326. doi:http://www.ncbi.nlm.nih.gov/pubmed/9351212
Papageorgiou S, Varvaresou A, Tsirivas E, Demetzos C (2010) New alternatives to cosmetics preservation. J Cosmet Sci 61:107–123
Bernatoniene J, Masteikova R, Davalgiene J, Bernatoniene J, Masteikova R, Davalgiene J, Peciura R, Gauryliene R, Bernatoniene R, Majiene D, Lazauskas R, Civinskiene G, Velziene S, Muselik J, Chalupova Z (2011) Topical application of Calendula officinalis (L.): formulation and evaluation of hydrophilic cream with antioxidant activity. J Med Plant Res 5:868–877
Author information
Authors and Affiliations
Corresponding author
Electronic supplementary material
Below is the link to the electronic supplementary material.
12088_2014_454_MOESM1_ESM.doc
Supplementary Table 1 Composition of the emulsions: E1–emulsion without methylparaben/essential oils/extracts; E2–emulsion with essential oils, E3–emulsion with extracts; E4–emulsion with methylparaben. (DOC 40 kb)
12088_2014_454_MOESM2_ESM.doc
Supplementary Table 2 Antibacterial and antifungal activity of essential oils, extracts and methylparaben (15 μL) in agar disc diffusion method, inhibition zones (mm). (DOC 43 kb)
Rights and permissions
About this article
Cite this article
Herman, A. Comparison of Antimicrobial Activity of Essential Oils, Plant Extracts and Methylparaben in Cosmetic Emulsions: 2 Months Study. Indian J Microbiol 54, 361–364 (2014). https://doi.org/10.1007/s12088-014-0454-z
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s12088-014-0454-z