Indian Journal of Microbiology

, Volume 54, Issue 1, pp 12–19 | Cite as

The Primary Research on the Gut Microbes in KKAy Mice

  • Yanqin LiEmail author
  • Liang Han
  • Ming Xu
  • Junjie Guo
  • Mali Feng
  • Xiaojiong Wang
Original Article


In the first part of this paper, gut microbial difference of two genotypes mice was researched. The gut microbial community of type 2 diabetes animal model KKAy mice and normal C57BL/6J mice had clear distinctions in DGGE (denaturing gradient gel electrophoresis) profiles. The pairwise similarity coefficient (C s ) was only 26–44 % between KKAy and C57BL/6J, but C s was 82–100 % among same genotypes mice. Thirteen dominant bands were cloned from DGGE profiles to exhibit difference on gut microbial structure further. In the second part of this paper, the influence of hypoglycemic drug Pioglitazone on the gut microbes in KKAy mice was researched by gut microbial diversity analysis and principal component analysis (PCA). The results showed that Pioglitazone reduced the gut microbial diversity slightly and changed gut microbial structure of KKAy mice to that of normal C57BL/6J mice.


Gut microbes KKAy mice Type 2 diabetes Genotypes Pioglitazone 



This work has been supported by the National Natural Science Foundation of China (30771310) and the Program of Science and Technology of Shanxi province, China (20120311006-1).


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Copyright information

© Association of Microbiologists of India 2013

Authors and Affiliations

  • Yanqin Li
    • 1
    Email author
  • Liang Han
    • 1
  • Ming Xu
    • 1
  • Junjie Guo
    • 2
  • Mali Feng
    • 2
  • Xiaojiong Wang
    • 2
  1. 1.Institute of BiotechnologyShanxi UniversityTaiyuanChina
  2. 2.Shanxi Hospital of Traditional Chinese MedicineTaiyuanChina

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