Abstract
The oral bacterium, Campylobacter rectus, is an etiological agent of periodontitis. The virulence genes of C. rectus are largely unknown. The aim of this study was to query C. rectus for the presence of an invasion antigen B (ciaB) gene, which is needed for cell invasion by the related species Campylobacter jejuni. PCR and PCR-walking identified a ciaB from C. rectus. In silico analyses of C. rectus 314 ciaB (Cr-ciaB) revealed an ORF of 1,830 base pairs. The Cr-CiaB protein shared significant sequence identity (BLASTx and phylogeny) with CiaB from related campylobacters. Cr-CiaB is predicted to lack membrane helices, signal peptides, and localizes to the cytoplasm; which are consistent with CiaB proteins. Expression of Cr-ciaB was confirmed with RT-PCR; and potential ciaB genes were detected in eight additional strains of C. rectus. Cr-ciaB is the first CiaB identified from the oral campylobacters.
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Acknowledgments
Michael J. LaGier was supported in part by Florida Gulf Coast University (Internal Grant #09101). This study was also supported in part by the NSF-POWRE MCB-9973861 to Deborah S. Threadgill.
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LaGier, M.J., Threadgill, D.S. Identification and Characterization of an Invasion Antigen B Gene from the Oral Pathogen Campylobacter rectus . Indian J Microbiol 54, 33–40 (2014). https://doi.org/10.1007/s12088-013-0406-z
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DOI: https://doi.org/10.1007/s12088-013-0406-z