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A Shortcut to the Optimization of Cellulase Production Using the Mutant Trichoderma reesei YC-108

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Abstract

Trichoderma reesei YC-108, a strain isolated by a kind of newly invented plate was found to over produce cellulase and it was then used as a cellulase producer. To get the maximum amount of cellulase, the combination of the medium ingredients, which has a profound influence on metabolic pathway was optimized using response surface methodology. The optimum composition was found to be 24.63 g/L wheat bran, 30.78 g/L avicel, and 19.16 g/L soya-bean cake powder. By using the optimized medium, the filter paper activity (FPA) increased nearly five times to 15.82 IU/mL in a 30 L stirred fermenter, carboxymethyl cellulase activity (CMCase) was increased from 83.02 to 628.05 IU/mL and the CMCase/FPA ratio was nearly doubled compared with the parent strain at initial medium.

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Abbreviations

X i , X j :

Independent variable coded value

Z j :

Independent variable real value, g/L

X 1 :

Coded value of wheat bran concentration

X 2 :

Coded value of avicel concentration

X 3 :

Coded value of soya-bean cake powder concentration

Y :

Predicted response (cellulase activity), IU/mL

b 0 :

Intercept term

b j :

Linear effect coefficient

b ij :

Interaction effect coefficient

b jj :

Squared effect coefficient

Δ j :

Step change value

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Acknowledgments

We wish to thank Yanli Fan and Yuou Teng for their excellent technical assistance. We are grateful to National natural science foundation of China (No: 31101357, 30900339)for financial support.

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Authors and Affiliations

  1. The Research Centre of Modern Analysis Technology, Tianjin University of Science & Technology, Tianjin, 300457, People’s Republic China

    Zhong-li Yan, Zhi-yan Yang & Juan Zhao

  2. College of Food Engineering and Biotechnology, Tianjin University of Science & Technology, Tianjin, 300457, People’s Republic China

    Xiao-hong Cao & Qing-dai Liu

  3. College of Biotechnology, Tianjin University of Science & Technology, Tianjin, 300457, People’s Republic China

    Yu-ou Teng

Authors
  1. Zhong-li Yan
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  2. Xiao-hong Cao
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  3. Qing-dai Liu
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  4. Zhi-yan Yang
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  5. Yu-ou Teng
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  6. Juan Zhao
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Correspondence to Zhong-li Yan.

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Yan, Zl., Cao, Xh., Liu, Qd. et al. A Shortcut to the Optimization of Cellulase Production Using the Mutant Trichoderma reesei YC-108. Indian J Microbiol 52, 670–675 (2012). https://doi.org/10.1007/s12088-012-0311-x

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  • DOI: https://doi.org/10.1007/s12088-012-0311-x

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