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Antibacterial Screening of Secreted Compounds Produced by the Phase I Variant of Photorhabdus luminescens

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Abstract

In this study, antibacterial activity of metabolites secreted by the phase I variant of Photorhabdus luminescens was investigated. Bioactivity of these metabolites was screened against 28 different bacterial species and strains. Bacterial sensitivity was determined by a modified-version of the Kirby–Bauer disk diffusion susceptibility method, whereas the phase I variant’s culture permeate was utilized as the “antibacterial” agent. This investigation demonstrates that 11 of the 28 bacterial species tested were sensitive to at least one of the secreted compounds or a combination thereof.

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Acknowledgments

Partial financial support for this study was provided in part by the Eunice Kennedy Shriver National Institute of Child Health and Development Grant 5G11HD052381-05 and the North Carolina Biotechnology Center Grant 2010-IDG-1008. The content of this article is solely the responsibility of the authors and does not necessarily represent the official views of the funding organizations.

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  1. Sartorius-Stedim Biotechnology Laboratory, Biotechnology Research and Training Center, University of North Carolina at Pembroke, 115 Livermore Drive, Pembroke, NC, 28372, USA

    Floyd L. Inman III & Leonard Holmes

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  1. Floyd L. Inman III
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  2. Leonard Holmes
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Correspondence to Floyd L. Inman III.

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Inman III, F.L., Holmes, L. Antibacterial Screening of Secreted Compounds Produced by the Phase I Variant of Photorhabdus luminescens . Indian J Microbiol 52, 708–709 (2012). https://doi.org/10.1007/s12088-012-0307-6

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  • DOI: https://doi.org/10.1007/s12088-012-0307-6

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