Skip to main content
SpringerLink
Log in

Genetic Variability of Beauveria bassiana and a DNA Marker for Environmental Monitoring of a Highly Virulent Isolate Against Cosmopolites sordidus

  • Original Article
  • Published:
Indian Journal of Microbiology Aims and scope Submit manuscript

Abstract

The banana weevil Cosmopolites sordidus (Germar) is one of a number of pests that attack banana crops. The use of the entomopathogenic fungus Beauveria bassiana as a biological control agent for this pest may contribute towards reducing the application of chemical insecticides on banana crops. In this study, the genetic variability of a collection of Brazilian isolates of B. bassiana was evaluated. Samples were obtained from various geographic regions of Brazil, and from different hosts of the Curculionidae family. Based on the DNA fingerprints generated by RAPD and AFLP, we found that 92 and 88 % of the loci were polymorphic, respectively. The B. bassiana isolates were attributed to two genotypic clusters based on the RAPD data, and to three genotypic clusters, when analyzed with AFLP. The nucleotide sequences of nuclear ribosomal DNA intergenic spacers confirmed that all isolates are in fact B. bassiana. Analysis of molecular variance showed that variability among the isolates was not correlated with geographic origin or hosts. A RAPD-specific marker for isolate CG 1024, which is highly virulent to C. sordidus, was cloned and sequenced. Based on the sequences obtained, specific PCR primers BbasCG1024F (5′-TGC GGC TGA GGA GGA CT-3′) and BbasCG1024R (5′-TGC GGC TGA GTG TAG AAC-3′) were designed for detecting and monitoring this isolate in the field.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3

Similar content being viewed by others

References

  1. Akello J, Dubois T, Gold CS, Coyne D, Nakavuma J, Paparu P (2007) Beauveria bassiana (Balsamo) Vuillemin as an endophyte in tissue culture banana (Musa spp.). J Invertebr Pathol 96:34–42

    Article  PubMed  Google Scholar 

  2. Gold CS, Pena JE, Karamura EB (2001) Biology and integrated pest management for the banana weevil, Cosmopolites sordidus (Germar) (Coleoptera: Curculionidae). Int Pest Manag Rev 6:79–155

    Article  Google Scholar 

  3. Sandhu SS, Sharma AK, Beniwal V, Goel G, Batra P, Kumar A, Jaglan S, Sharma AK, Malhotra S (2012) Myco-biocontrol of insect pests: factors involved, mechanism, and regulation. J Pathog. doi:10.1155/2012/126819

  4. Oliveira FQ, Malaquias JB, Ferreira LL, Ribeiro TS, Pereira AIA (2010) Efficacy of molasses and Beauveria bassiana (Balsamo) Vuill on the control of Cosmopolites sorditus Germar, 1824. Eng Ambient 7:127–132

    Google Scholar 

  5. Rehner SA, Minnis AM, Sung GH, Luangsa-ard JJ, Devotto L, Humber RA (2011) Phylogeny and systematics of the anamorphic, entomopathogenic genus Beauveria. Mycologia 103:1055–1073

    Article  PubMed  Google Scholar 

  6. Castrillo LA, Vandenberg JD, Wraight SP (2003) Strain-specific detection of introduced Beauveria bassiana in agricultural fields by use of sequence-characterized amplified region markers. J Invertebr Pathol 82:75–83

    Article  PubMed  CAS  Google Scholar 

  7. Meyling NV, Lübeck M, Buckley EP, Eilenberg J, Rehner SA (2009) Community composition, host range and genetic structure of the fungal entomopathogen Beauveria in adjoining agricultural and seminatural habitats. Mol Ecol 18:1282–1293

    Article  PubMed  CAS  Google Scholar 

  8. Wang C, Fan M, Li Z, Butt TM (2004) Molecular monitoring and evaluation of the application of the insect-pathogenic fungus Beauveria bassiana in southeast China. J Appl Microbiol 96:861–870

    Article  PubMed  CAS  Google Scholar 

  9. Pontecorvo G, Roper JA, Hemmons LM, Macdonald KD, Bufton AWJ (1953) The genetics of Aspergillus nidulans. Adv Gen 5:141–238

    Article  CAS  Google Scholar 

  10. Fungaro MHP, Vieira MLC, Pizzirani-Kleiner AA, Azevedo JL (1996) Diversity among soil and insect isolates of Metarhizium anisopliae var. anisopliae detected by RAPD. Lett Appl Microbiol 22:389–392

    Article  Google Scholar 

  11. Munhoz CF, Weiss B, Hanai LR, Zucchi MI, Fungaro MHP, Oliveira ALM, Monteiro-Vitorello CB, Vieira MLC (2011) Genetic diversity and a PCR-based method for Xanthomonas axonopodis detection in passion fruit. Phytopathology 101:416–424

    Article  PubMed  CAS  Google Scholar 

  12. Vos P, Hogers R, Bleeker M, Reijans M, Vandelee T, Hornes M, Frijters A, Pot J, Peleman J, Kuiper M, Zabeau M (1995) AFLP: a new technique for DNA fingerprinting. Nucleic Acids Res 23:4407–4414

    Article  PubMed  CAS  Google Scholar 

  13. White TJ, Burns T, Lee S, Taylor J (1990) Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. In: Innis MA, Gelfand DH, Sninsky JJ, White TJ (eds) PCR protocols: a guide to methods and applications. Academic Press, San Diego, pp 315–322

    Google Scholar 

  14. Tamura K, Peterson D, Peterson N, Stecher G, Nei M, Kumar S (2011) MEGA5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. Mol Biol Evol 28:2731–2739

    Article  PubMed  CAS  Google Scholar 

  15. Saitou N, Nei M (1987) The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4:406–425

    PubMed  CAS  Google Scholar 

  16. Schneider S, Kueffer JM, Roessli D, Excoffier L (2000) ARLEQUIN version 2.000. A software for population genetic data analysis. Genetic and Biometry Laboratory, University of Geneva, Switzerland

    Google Scholar 

  17. Pritchard JK, Stephens M, Donnelly P (2000) Inference of population structure using multilocus genotype data. Genetics 155:945–959

    PubMed  CAS  Google Scholar 

  18. Evano G, Regnaut S, Goudet J (2005) Detecting the number of clusters of individuals using the software structure: a simulation study. Mol Ecol 14:2611–2620

    Article  Google Scholar 

  19. Wang C, Shah FA, Patel N, Li ZZ, Butt TM (2003) Molecular investigation on strain genetic relatedness and population structure of Beauveria bassiana. Environ Microbiol 10:908–915

    Article  Google Scholar 

  20. Castrillo LA, Bauer LS, Liu H, Griggs MH, Vandenberg JD (2010) Beauveria bassiana (Ascomycota: Hypocreales) isolates associated with Agrilus planipennis (Coleoptera: Buprestidae) populations in Michigan. Biol Control 54:135–140

    Article  Google Scholar 

  21. Meyling NV, Pilz C, Keller S, Widmer F, Enkerli J (2012) Diversity of Beauveria spp. isolates from pollen beetles Meligethes aeneus in Switzerland. J Invertebr Pathol 109:76–82

    Article  PubMed  Google Scholar 

  22. Ghikas DV, Kouvelis VN, Typas MA (2010) Phylogenetic and biogeographic implications inferred by mitochondrial intergenic region analyses and ITS1-5.8S-ITS2 of the entomopathogenic fungi Beauveria bassiana and B. brongniartii. BMC Microbiol 10:174–189

    Article  PubMed  Google Scholar 

  23. Garrido-Jurado I, Márquez M, Ortiz-Urquiza A, Santiago-Álvarez C, Iturriaga EA, Quesada-Moraga E, Monte E, Hermosa R (2011) Genetic analyses place most Spanish isolates of Beauveria bassiana in a molecular group with word-wide distribution. BMC Microbiol 11:84–95

    Article  PubMed  CAS  Google Scholar 

  24. Lopes RB, Michereff-Filho M, Tigano MS, Neves PMOJ, López EL, Fancelli M, da Silva JP (2011) Virulence and horizontal transmission of selected Brazilian strains of Beauveria bassiana against Cosmopolites sordidus under laboratory conditions. B Insectol 64:201–208

    Google Scholar 

  25. Dissertation. Lema EL (2010). Biological control of Cosmopolites sordidus (Germar) (Coleoptera: Dryophthoridae) with Beauveria bassiana (Bals.) Vuill. And population fluctuation in Ibiporã, Brazil. University of Londrina

Download references

Acknowledgments

This work was supported by grants and fellowships from the Brazilian institutions, Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) and Coordenadoria de Aperfeiçoamento de Pessoal do Ensino Superior (CAPES).

Author information

Authors and Affiliations

  1. Departamento de Biologia Geral, Universidade Estadual de Londrina, P.O. Box 6001, Londrina, 86051-970, Brazil

    D. V. Ferri, P. M. O. Neves, L. M. Ferracin, D. Sartori, M. L. C. Vieira & M. H. P. Fungaro

  2. Departamento de Genética, Escola Superior de Agricultura “Luiz de Queiroz”, Universidade de São Paulo, P.O. Box 83, Piracicaba, 13400-970, Brazil

    C. F. Munhoz

Authors
  1. D. V. Ferri
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. C. F. Munhoz
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. P. M. O. Neves
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. L. M. Ferracin
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. D. Sartori
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. M. L. C. Vieira
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. M. H. P. Fungaro
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to M. H. P. Fungaro.

Electronic supplementary material

Rights and permissions

Reprints and permissions

About this article

Cite this article

Ferri, D.V., Munhoz, C.F., Neves, P.M.O. et al. Genetic Variability of Beauveria bassiana and a DNA Marker for Environmental Monitoring of a Highly Virulent Isolate Against Cosmopolites sordidus . Indian J Microbiol 52, 569–574 (2012). https://doi.org/10.1007/s12088-012-0292-9

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s12088-012-0292-9

Keywords

Search

Navigation