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Cloning of a Family 11 Xylanase Gene from Bacillus amyloliquefaciens CH51 Isolated from Cheonggukjang

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Abstract

Bacillus amyloliquefaciens CH51, an isolate from cheonggukjang, Korean fermented soyfood, secretes several enzymes into culture medium. A gene encoding 19 kDa xylanase was cloned by PCR. Sequencing showed that the gene encoded a glycohydrolase family 11 xylanase and named xynA. xynAHis, xynA with additional codons for his-tag, was overexpressed in Escherichia coli BL21(DE3) using pET-26b(+). XynAHis was purified using HisTrap affinity column. Km and Vmax of XynAHis were 0.363 mg/ml and 701.1 μmol/min/mg, respectively with birchwood xylan as a substrate. The optimum pH and temperature were pH 4 and 25 °C, respectively. When xynA was introduced into Bacillus subtilis WB600, active XynA was secreted into culture medium.

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Acknowledgments

This work was supported by a grant from Ministry of Knowledge Economy, Korea. C. Baek and S. Lee were supported by BK21 program from MEST, Korea.

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Authors and Affiliations

  1. Division of Applied Life Science (Bk21), Graduate School, Research Institute of Life Sciences, Gyeongsang National University, Jinju, Korea

    C. U. Baek, S. G. Lee, Y. R. Chung & J. H. Kim

  2. Energy R&D center, SK energy, Daejeon, Korea

    I. Cho

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  1. C. U. Baek
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  2. S. G. Lee
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  3. Y. R. Chung
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  4. I. Cho
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  5. J. H. Kim
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Correspondence to J. H. Kim.

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Baek, C.U., Lee, S.G., Chung, Y.R. et al. Cloning of a Family 11 Xylanase Gene from Bacillus amyloliquefaciens CH51 Isolated from Cheonggukjang. Indian J Microbiol 52, 695–700 (2012). https://doi.org/10.1007/s12088-012-0260-4

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  • DOI: https://doi.org/10.1007/s12088-012-0260-4

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