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Detection of Mycobacterium bovis and Mycobacterium tuberculosis from Cattle: Possible Public Health Relevance

Abstract

Mycobacterium bovis and Mycobacterium tuberculosis infect both animals and humans. The disease epidemiology by these agents differs in developed and developing countries due to the differences in the implementation of the prevention and control strategies. The present study describes the detection of M. bovis and M. tuberculosis from specimens of lungs and pulmonary lymph nodes of four cattle died in an organized herd of 183 cattle in the state of Himachal Pradesh, India, with inconclusive skin test results. Identification and distinction of these closely related mycobacterial species was done by PCR-RFLP targeting hsp65 gene followed by spacer oligonucleotide typing. Mixed infection of M. bovis and M. tuberculosis was detected in one cattle.

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Acknowledgments

Authors are thankful to Dr. V.M. Katoch, Secretary, Department of Health Research, Govt. of India and Director-General ICMR (Ex-Director NJIL & OMD, Agra, India) for support and giving access to BSL-3 facility at the institute.

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Correspondence to Aneesh Thakur.

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Thakur, A., Sharma, M., Katoch, V.C. et al. Detection of Mycobacterium bovis and Mycobacterium tuberculosis from Cattle: Possible Public Health Relevance. Indian J Microbiol 52, 289–291 (2012). https://doi.org/10.1007/s12088-011-0200-8

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  • DOI: https://doi.org/10.1007/s12088-011-0200-8

Keywords

  • M. bovis
  • M. tuberculosis
  • Cattle
  • Public health
  • Spoligotyping